2018 Fiscal Year Annual Research Report
メッセンジャーRNAを安定化する高分子材料の設計と生体内デリバリーヘの挑戦
| Project/Area Number |
18F18115
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| Research Institution | The University of Tokyo |
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Principal Investigator |
宮田 完二郎 東京大学, 大学院工学系研究科(工学部), 准教授 (50436523)
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| Co-Investigator(Kenkyū-buntansha) |
KIM BEOB SOO 東京大学, 工学(系)研究科(研究院), 外国人特別研究員
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| Project Period (FY) |
2018-04-25 – 2020-03-31
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| Keywords | Cationic polymer / Messenger RNA / Transfection / Amphiphile |
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| Outline of Annual Research Achievements |
The research purpose is to synthesize new amphiphilic polymers for efficient mRNA transfection in immortal T cells (Jurkat cells). Precursor polymers, poly(beta-benzyl-L-aspartate) (PBLA) with a degree of polymerization (DP) 21 was prepared by a ring-opening polymerization.The previous results demonstrated that the cyclohexylethyl amine (CHE) and diethylenediamine (DET) moieties-bearing amphiphilic polymer induced high messenger RNA (mRNA) transfection efficacy in vitro. Accordingly, new aliphatic amines with ring structure were selected here as next candidate moieties. The new aliphatic amines and DET were simultaneously reacted with PBLA. The obtained results showed that the introduction ratio of the aliphatic amines was lower than expected, suggesting that the synthesis condition needs to be optimized in the next fiscal year.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
当初予定していたポリマーの合成が完了しなかったため。
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| Strategy for Future Research Activity |
The synthesis of amphiphilic polymers was little bit slower than the initial schedule because the previous synthetic methods did not provide sufficient introduction rate of hydrophobic moiety. This is because some aliphatic amines did not have a sufficient purity, resulting in the low reactivity with the precursor polymer. The polymer synthesis will further be optimized to obtain proper hydrophobic introduction rates. The feeding ratio and reaction time will be further altered to increase the introduction raties of hydrophobic moiety.
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Research Products
(4 results)
