Elucidation of roles of gasotransmitters in the pathogenesis of atopic dermatitis

2020 Fiscal Year Annual Research Report

• Project/Area Number: 18F18410
• Research Institution: Juntendo University
• Principal Investigator: 高森 建二 順天堂大学, 医学部, 特任教授 (40053144)
• Co-Investigator(Kenkyū-buntansha): MONIAGA CATHARINA 順天堂大学, 医学部, 外国人特別研究員
• Project Period (FY): 2018-10-12 – 2021-03-31
• Keywords: Hydrogen sulfide / Atopic dermatitis / axon-guidance molecules / human keratinocytes

Outline of Annual Research Achievements

Despite the numerous reports that H2S is involved in various pathological diseases and/or itch, however, there have been no reports of its involvement in the expression of axon-guidance molecules in human keratinocytes (KCs).I found that H2S-producing enzymes were expressed in human skin (mainly KCs), and were increased in human atopic dermatitis (AD) skin lesion. Notably, the H2S donor stimulation upregulated NGF and ARTN, and downregulated Sema3A levels in the cultured normal human epidermal keratinocytes, which is considered to be a cause of peripheral itch sensitization in AD skin lesion. I suggest that the involvement of H2S under the experimental conditions used in the present work in relation with AD. Our data raise the possibility that H2S, at least in part, modulates the epidermal hyperinnervation of the human skin through alteration of axon-guidance molecules in KCs. These findings add insight of the possible role of gasotransmitter in epidermal hyperinnervation of human skin, especially in itchy skin inflammatory condition such as AD. The result of the project has been published in international journal (Journal of Dermatological Science).

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Mechanisms and Management of Itch in Dry Skin (2020)
  • Author(s): Moniaga CS, Tominaga M, Takamori M.
  • Journal Title: Acta Derm Venereol Volume: 100 Pages: -
  • DOI: 10.2340/00015555-3344.
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hydrogen sulfide modulates the expression of axon-guidance molecules in human keratinocytes (2020)
  • Author(s): Moniaga CS, Kamata Y, Ogawa H, Suga Y, Tominaga M, Takamori K.
  • Journal Title: J Dermatol Sci. Volume: 97 Pages: 232-235
  • DOI: 10.1016/j.jdermsci.2020.01.007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Calcium-Inducible MAPK/AP-1 Signaling Drives Semaphorin 3A Expression in Normal Human Epidermal Keratinocytes. (2020)
  • Author(s): Kamata Y, Tominaga M, Umehara Y, Honda K , Kamo A, Moniaga CS , Komiya E, Toyama S , Suga Y , Ogawa H, Takamori K
  • Journal Title: J Invest Dermatol. Volume: 140 Pages: 1346-1354
  • DOI: 10.1016/j.jid.2020.01.001