2019 Fiscal Year Annual Research Report
量子ドットによる新規セラノスティックナノ粒子の開発
| Project/Area Number |
18F18766
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| Research Institution | National Institutes for Quantum and Radiological Science and Technology |
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Principal Investigator |
青木 伊知男 国立研究開発法人量子科学技術研究開発機構, 量子生命科学領域, 統括グループリーダー(定常) (10319519)
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| Co-Investigator(Kenkyū-buntansha) |
SEMKOVA SEVERINA 国立研究開発法人量子科学技術研究開発機構, 分子イメージング診断治療研究部・機能分子計測グループ, 外国人特別研究員
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| Project Period (FY) |
2018-10-12 – 2020-03-31
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| Keywords | theranostics / quantum dots / nitroxide / imaging |
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| Outline of Annual Research Achievements |
We developed new theranostic nanoparticles, based on quantum dots (QDs), conjugated with multispin nitroxide-cyclodextrins (QD@CD-TEMPO and QD@CD-TEMPOH) and triphenylphosphonium (TPP). Both sensors are applicable for quantitative imaging assays of oxidative stress and redox-status of living cells and tissues.
These nanoparticles combine several features: (i) three imaging modalities (MRI, EPR, optical); (ii) intracellular delivery and localization mainly into the mitochondria due to TPP; (iii) possibility to sense and visualize the areas of oxidative stress and redox-status in living cells and tissues – due to dynamics of their contrast properties, based on the balance between oxidizers and reducers; (iv) redox-modulating activity and possibility to induce apoptosis in cancer cells, without significant effect on viability of normal cells due to SOD-mimetic feature of nitroxide residues.
The analytical tests using both sensors were validated by conventional redox analyses (mitoSOX, DHE, DCF, TAC).
Both sensors were applied for:
1. EPR/optical imaging of oxidative stress and total reducing capacity in cultured cells with exogenously induced mitochondrial dysfunction. Cytotoxicity and induction of apoptosis on normal and cancer cells were also analysed (MTS assay, Annexin-V test).
2. MR imaging in animals with kidney dysfunction, induced by oxidative stress.
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| Research Progress Status |
令和元年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和元年度が最終年度であるため、記入しない。
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