Molecular basis of regulation of organ size-Molecular genetic approaches to reveal mechanisms of compensation

2010 Fiscal Year Final Research Report

• Project/Area Number: 18GS0313
• Research Category: Grant-in-Aid for Creative Scientific Research
• Allocation Type: Single-year Grants
• Research Institution: The University of Tokyo
• Principal Investigator: TSUKAYA Hirokazu 東京大学, 大学院・理学系研究科, 教授 (90260512)
• Co-Investigator(Kenkyū-buntansha): HORIGUCHI Goro 立教大学, 理学部・生命理学科, 准教授 (70342847) ; YAMAGUCHI Takahiro 東京大学, 大学院・理学系研究科, 特任研究員 (60450201) ; FERJANI Ali 東京学芸大学, 教育学部, 助教 (20530380)
• Project Period (FY): 2006 – 2010
• Keywords: 器官サイズ / 発生・分化 / 葉 / 補償作用

Research Abstract

Our studies on mechanisms of leaf development have revealed that an unknown system integrates activities of cell proliferation and cell expansion in leaves, depending on non-cell autonomous regulations. We also showed that gene expression not only regulates leaf form, but the resulting organ shape also secondarily regulates gene expression in a particular part of the organ primordium. These findings strongly indicate importance of understanding of the organ-level integration of cell behaviors to reveal mechanisms of organogenesis in multicellular organs.

Research Products

• [Journal Article] ROTUNDIFOLIA4 regulates cellproliferation along the body axis in Arabidopsis shoot (2011)
  • Author(s): Ikeuchi M, Yamaguchi T, Kazama T, Ito T, Horiguchi G, and Tsukaya H
  • Journal Title: Plant Cell Physiol Volume: 52 Pages: 59-69
  • Peer Reviewed
• [Journal Article] ANGUSTIFOLIA3 plays roles inadaxial/abaxial patterning and growth in leaf morphogenesis (2011)
  • Author(s): Horiguchi G, Nakayama H, Ishikawa N, Kubo M, Demura T, Fukuda H, and Tsukaya H
  • Journal Title: Plant Cell Physiol Volume: 52 Pages: 112-124
  • Peer Reviewed
• [Journal Article] Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development (2011)
  • Author(s): Horiguchi G, Molla-Morales A, Perez-Perez JM, Kojima K, Robles P, Ponce MR, Micol JL, and Tsukaya H
  • Journal Title: Plant J Volume: 65 Pages: 724-736
  • Peer Reviewed
• [Journal Article] Keep aneye on PPi : The vacuolar-type H^+-pyrophosphatase regulates post-germinative development in Arabidopsis (2011)
  • Author(s): Ferjani A, Segami S, Horiguchi G, MutoY, Maeshima M, Tsukaya H
  • Journal Title: Plant Cell Volume: 23 Pages: 2895-2908
  • Peer Reviewed
• [Journal Article] Key proliferative activity in the junction between the leaf blade and the leaf petiole of Arabidopsis thaliana (2011)
  • Author(s): Ichihashi Y, Kawade K, Usami T, Horiguchi G, Takahashi T, Tsukaya H
  • Journal Title: Plant Phys Volume: 157 Pages: 1151-1162
  • Peer Reviewed
• [Journal Article] AUNGUSTIFOLIA, a plant homolog of CtBP/BARS, functions outside the nucleus (2011)
  • Author(s): Minamisawa N, Sato M, Cho K-H, Ueno H, Takeuchi K, Kajiwara M, Yamato KT, Ohyama K, Toyooka K, Kim G-T, Horiguchi G, Takano H, Ueda T, and Tsukaya H
  • Journal Title: Plant J Volume: 68 Pages: 788-799
  • Peer Reviewed
• [Journal Article] Plant Elongator regulates auxin-related genes during RNA polymeraseII transcription elongation (2010)
  • Author(s): Nelissen H., Fleury D., De Groeve, S., Bruno, L., Yamaguchi, T., Prinsen E., Cnops, G., Neyt, P., Bitonti, B., De Block, M., Witters, E., De Jaeger, G., Tsukaya, H., Houben, A. and Van Lijsebettens M
  • Journal Title: Proc. Natl. Acad. Sci. Volume: 107 Pages: 1678-1683
  • Peer Reviewed
• [Journal Article] The bHLH transcription factor SPATULA controls final leaf size in Arabidopsis thaliana (2010)
  • Author(s): Ichihashi Y, Horiguchi G, Gleissberg S, Tsukaya H
  • Journal Title: Plant Cell Physiol Volume: 51 Pages: 252-261
  • Peer Reviewed
• [Journal Article] Mechanisms of leaf tooth formation in Arabidopsis (2010)
  • Author(s): Kawamura E, Horiguchi G, Tsukaya H
  • Journal Title: Plant J Volume: 62 Pages: 429-441
  • Peer Reviewed
• [Journal Article] The mechanism of cell cyclearrest front progression explained by a KLUH/CYP78A5-dependent mobile growth factor in developing leaves of Arabidopsis thaliana (2010)
  • Author(s): Kazama T, Ichihashi Y, Murata S, Tsukaya H
  • Journal Title: Plant Cell Physiol Volume: 51 Pages: 1046-1054
  • Peer Reviewed
• [Journal Article] Characterization of EMU, the Arabidopsis homolog of the yeast THO complex member HPR1 (2010)
  • Author(s): Furumizu C, Tsukaya H, Komeda Y
  • Journal Title: RNA Volume: 16 Pages: 1809-181
  • Peer Reviewed
• [Journal Article] Non-cell-autonomously coordinated organ-size regulation in leaf development (2010)
  • Author(s): Kawade K, Horiguchi G and Tsukaya H
  • Journal Title: Development Volume: 137 Pages: 4221-4227
  • Peer Reviewed
• [Journal Article] The more and smaller cells mutants of Arabidopsis thaliana identify novel roles for SQUAMOSA PROMOTER BINDING PROTEIN-LIKE genes in the control of heteroblasty (2009)
  • Author(s): Usami T, Horiguchi G, Yano S and Tsukaya H
  • Journal Title: Development Volume: 136 Pages: 955-964
  • Peer Reviewed
• [Journal Article] Coordination of cell proliferation and cell expansion mediated by ribosome-related processes in the leaves of Arabidopsis thaliana (2009)
  • Author(s): Fujikura U, Horiguchi G, Ponce MR, Micol, JL, and Tsukaya H
  • Journal Title: Plant J Volume: 59 Pages: 499-508
  • Peer Reviewed
• [Journal Article] Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana (2009)
  • Author(s): Horiguchi G, Gonzalez N, Beemster GTS, Inze D, and Tsukaya H
  • Journal Title: Plant J Volume: 60 Pages: 122-133
  • Peer Reviewed
• [Journal Article] Isolation and characterization of the Larix gmelini ANGUSTIFOLIA(LgAN) gene (2008)
  • Author(s): Lin X, Minamisawa N, Takechi K, Zhang W, Sato H, Takio S, Tsukaya H and Takano H
  • Journal Title: Planta Volume: 228 Pages: 601-608
  • Peer Reviewed
• [Journal Article] AtMap1 : a DNA Microarray for Genomic Deletion Mapping in Arabidopsis thaliana (2008)
  • Author(s): Nagano AJ, Fukasawa M., Hayashi M., Ikeuchi M., Tsukaya H, Nishimura M., Hara-Nishimura I
  • Journal Title: Plant J Volume: 56 Pages: 1058-1065
  • Peer Reviewed
• [Journal Article] Dissection of enhanced cell expansion processes in leaves triggered by defect in cell proliferation, with reference to roles of endoreduplication (2007)
  • Author(s): Fujikura U., Horiguchi, G., Tsukaya, H
  • Journal Title: Plant Cell Physiol Volume: 48 Pages: 278-286
  • Peer Reviewed
• [Journal Article] Analysis of leaf development in fugu mutants of Arabidopsis reveals three compensation modes that modulate cell expansion in determinate organs (2007)
  • Author(s): Ferjani, A., Satoshi, Y., Horiguchi, G., and Tsukaya, H
  • Journal Title: Plant Physiol Volume: 144 Pages: 988-999
  • Peer Reviewed
• [Journal Article] Genetic relationship between angustifolia3 and extra-small sisters highlights novel mechanisms controlling leaf size (2007)
  • Author(s): Fujikura U, Horiguchi G, Tsukaya H
  • Journal Title: Plant Signaling & Behavior Volume: 2(5) Pages: 378-380
  • Peer Reviewed
• [Journal Article] DRL regulates adaxial leaf patterning and shoot apical meristem activity in Arabidopsis (2007)
  • Author(s): Cho, K.-H., Choi, H., Seki, M., Jun, S. E., Yi, Y. B., Shinozaki, K., Tsukaya, H., andKim, G.-T
  • Journal Title: J. Plant Biol Volume: 50 Pages: 467-474
  • Peer Reviewed
• [Journal Article] Structurally related Arabidopsis ANGUSTIFOLIA is functionally distinct from the transcriptional corepressor CtBP (2007)
  • Author(s): Stern MD, Aihara H, Cho K-H, Kim G-T, Horiguchi G, Roccaro GA, Guevara E, Sun J, Negeri D, Tsukaya H, Nibu Y
  • Journal Title: Dev, Genes & Evol Volume: 217 Pages: 759-769
  • Peer Reviewed
• [Journal Article] BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis (2007)
  • Author(s): Breuer C, Stacey NJ, Roberts G, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K
  • Journal Title: Plant Cell Volume: 19 Pages: 3655-3668
  • Peer Reviewed
• [Journal Article] Coordination of cell proliferation and cell expansion in the control of leaf size in Arabidopsis thaliana (2006)
  • Author(s): Horiguchi G, Ferjani A, Fujikura U, and Tsukaya H
  • Journal Title: J. Plant Res Volume: 119 Pages: 37-42
  • Peer Reviewed
• [Journal Article] Large-scale histological analysis of leaf mutants using two simple leaf observation methods : identification of novel genetic pathways governing the size shape of leaves (2006)
  • Author(s): Horiguchi G, Fujikura U, Ferjani A, Ishikawa N, Tsukaya H
  • Journal Title: Plant J Volume: 48 Pages: 638-644
  • Peer Reviewed
• [Presentation] Inter-cell-layer movement of ANGUSTIFOLIA3 coordinates cell proliferation between clonally distinct tissues in leaves (2011)
  • Author(s): Kensuke Kawade
  • Organizer: XVIII INTERNATIONAL BOTANICAL CONGRESS 2011
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2011-07-29
• [Presentation] Removal of Cytosolic PPi is a Prerequisite for Proper Resumption of Post-Germinative Development in Arabidopsis thaliana (2011)
  • Author(s): Ali Ferjani
  • Organizer: XVIII INTERNATIONAL BOTANICAL CONGRESS 2011
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2011-07-29
• [Presentation] Developmentalbiologists and housekeeping genes : the whys and wherefores (2011)
  • Author(s): Hirokazu Tsukaya
  • Organizer: XVIII INTERNATIONAL BOTANICAL CONGRESS 2011
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2011-07-28
• [Presentation] General and specific roles of the ribosome in leaf development (2011)
  • Author(s): Gorou Horiguchi
  • Organizer: XVIII INTERNATIONAL BOTANICAL CONGRESS 2011
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2011-07-28
• [Presentation] CHARACTERIZATION OF INTERCELLULAR SIGNALING THAT COORDINATES CELL PROLIFERATION WITH POST-MITOTIC CELL EXPANSION DURING LEAF DEVELOPMENT (2010)
  • Author(s): Kensuke Kawade
  • Organizer: 21^<st> International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 20100606-10
• [Presentation] Integration of cell proliferation and cell expansion in leaf organogenesis (2010)
  • Author(s): Hirokazu Tsukaya
  • Organizer: Regulation of Plant Growth from CELLS to ORGANS to ORGANISMS
  • Place of Presentation: Universitat Potsdam, Potsdam, Germany
  • Year and Date: 2010-04-13
• [Presentation] Integration of local information on leaf organogenesis (2009)
  • Author(s): Tsukaya, H
  • Organizer: Plant Growth Biology and Modeling workshop
  • Place of Presentation: Spain
  • Year and Date: 2009-10-14
• [Presentation] Leaf size is regulated by a cell-autonomous system linking cell proliferation and post-mitotic cell enlargement (2009)
  • Author(s): Kensuke Kawade
  • Organizer: 20^<th> International Conference on Arabidopsis Research
  • Place of Presentation: Edinburgh, Scotland, United Kingdom
  • Year and Date: 2009-07-01
• [Presentation] Genetic Basis of Leaf Shape/Size Evolution (2008)
  • Author(s): Hirokazu Tsukaya
  • Organizer: The 8^<th> NIBB-EMBL Joint Meeting Evolution : Genomes, Cell Types and Shapes
  • Place of Presentation: Okazaki Conference Center
  • Year and Date: 20081121-23
• [Presentation] Effect of sucrose on compensated cell enlargement in fugu5 mutant cotyledons (2008)
  • Author(s): Ferjani, Ali
  • Organizer: 19^<th> International Conference on Arabidopsis Research
  • Place of Presentation: Montreal, Canada
  • Year and Date: 20080723-27
• [Presentation] Gdp1, a putative RNA-binding protein, participates in the control of leaf shape and cell proliferation (2008)
  • Author(s): Horiguchi, Gorou
  • Organizer: 19^<th> International Conference on Arabidopsis Research
  • Place of Presentation: Montreal, Canada
  • Year and Date: 20080723-27
• [Presentation] Developmental analysis on the tooth in Arabidopsis (2008)
  • Author(s): Kawamura, Eiko
  • Organizer: 19^<th> International Conference on Arabidopsis Research
  • Place of Presentation: Montreal, Canada
  • Year and Date: 20080723-27
• [Presentation] Unknown relationship between leaf-cell size and ploidy level (2008)
  • Author(s): H. Tsukaya
  • Organizer: 19^<th> International Conference on Arabidopsis Research
  • Place of Presentation: Montreal, Canada
  • Year and Date: 20080723-27
• [Presentation] Evolutionary Developmental Studies on Unifacial Leaves in Monocots (2008)
  • Author(s): Takahiro Yamaguchi
  • Organizer: The 55^<th> NIBB Conference Arabidopsis Workshop 2008 "Frontiers of Plant Sciencein the 21^<st> Century"
  • Place of Presentation: Okazaki Conference Center
  • Year and Date: 2008-09-13
• [Presentation] FUGU5/AVP1 Activity Is Essential for Post-Germinative Growth and Organ-Size Control in Arabidopsis (2008)
  • Author(s): Ali Ferjani
  • Organizer: The 55^<th> NIBB Conference Arabidopsis Workshop 2008 "Frontiers of Plant Sciencein the 21^<st> Century"
  • Place of Presentation: Okazaki Conference Center
  • Year and Date: 2008-09-13
• [Presentation] ANGUSTIFOLIA3 Plays a Role in the Growth and Patterning of Leaf through Nucleolar Functions (2008)
  • Author(s): Gorou Horiguchi
  • Organizer: The 55^<th> NIBB Conference Arabidopsis Workshop 2008 "Frontiers of Plant Sciencein the 21^<st> Century"
  • Place of Presentation: Okazaki Conference Center
  • Year and Date: 2008-09-13
• [Presentation] The more and smallercells Mutants of Arabidopsis thaliana Identify Novel Roles of SQUAMOSA PROMOTE RBINDING PROTEIN-LIKE Genes in the Control of Heteroblasty (2008)
  • Author(s): Takeshi Usami
  • Organizer: The 55^<th> NIBB Conference Arabidopsis Workshop 2008 "Frontiers of Plant Sciencein the 21^<st> Century"
  • Place of Presentation: Okazaki Conference Center
  • Year and Date: 2008-09-13
• [Presentation] Developmental Analysis of Tooth in Arabidopsis (2008)
  • Author(s): Eiko Kawamura
  • Organizer: The 55^<th> NIBB Conference Arabidopsis Workshop 2008 "Frontiers of Plant Sciencein the 21^<st> Century"
  • Place of Presentation: Okazaki Conference Center
  • Year and Date: 2008-09-13
• [Presentation] Leaf Meristem : Meristematic Region that Supplies Cells Bidirectionally to Form Leaf Blade and Petiole (2008)
  • Author(s): Yasunori Ichihashi
  • Organizer: The 55^<th> NIBB Conference Arabidopsis Workshop 2008 "Frontiers of Plant Sciencein the 21^<st> Century"
  • Place of Presentation: Okazaki Conference Center
  • Year and Date: 2008-09-13
• [Presentation] 細胞増殖停止と生命現象植物の葉における細胞増殖停止と細胞伸長 (2007)
  • Author(s): 塚谷裕一
  • Organizer: 第30回日本分子生物学会年会第80回日本生化学大会合同大会
  • Place of Presentation: パシフィコ横浜ヨコハマグランドインターコンチネンタルホテル
  • Year and Date: 20071211-15
• [Presentation] Too few cells are toomuch for the plant : organ-wide regulationof leaf size (2007)
  • Author(s): Gorou Hoiguchi
  • Organizer: FASEB summer research conferences
  • Place of Presentation: Vermont
  • Year and Date: 20070812-17
• [Presentation] Organ size, cell size and ploidy : how an increase in ploidy promotes cell and organ growth? (2007)
  • Author(s): Hirokazu Tsukaya
  • Organizer: 18th International Conference of Arabidopsis Reserch
  • Place of Presentation: Beijing, China
  • Year and Date: 20070620-23
• [Presentation] Mechanisms of leaf size evolution in terms of ploidy level (2007)
  • Author(s): Hirokazu Tsukaya
  • Organizer: Pre-Symposium of East Asian Society of Plant Taxonomists "Evo-Devo & Phylogenetics, Regional Floristic Studies"
  • Place of Presentation: Chungbuk National University, Chung-Ju, Korea
  • Year and Date: 2007-10-05
• [Presentation] How leaf size and shape are controlled? Genetic analyses of Arabidopsis mutants as an attempt toanswer the question (2007)
  • Author(s): Tsukaya, Hirokazu
  • Organizer: Plant Biology & Botany 2007JOINT CONGRESS
  • Place of Presentation: Hilton Chicago, U. S. A
  • Year and Date: 2007-07-09
• [Presentation] 葉の器官サイズの制御と補償作用:多細胞系における細胞増殖と細胞伸長との統合システ (2006)
  • Author(s): 塚谷裕一
  • Organizer: 日本分子生物学会2006フォーラム
  • Place of Presentation: 名古屋国際会議場名古屋市
  • Year and Date: 20061206-08
• [Presentation] Coordination of cell proliferation and cell expansion processes in leaf size determination (2006)
  • Author(s): Ali Ferjani
  • Organizer: XV Congress of the Federation of European Societies of Plant Biology
  • Place of Presentation: Lyon
  • Year and Date: 20060717-21
• [Presentation] Organ-level control of cell proliferation/enlargement in leaf morphogenesis (2006)
  • Author(s): 塚谷裕一
  • Organizer: CDB Symposium 2006理化学研究所発生・再生科学総合研究センター
  • Place of Presentation: 神戸市
  • Year and Date: 20060410-12
• [Presentation] Cross-talks between cell proliferation and cell expansion in leaf development (2006)
  • Author(s): Ali Ferjani
  • Organizer: CDB Symposium 2006理化学研究所発生・再生科学総合研究センター
  • Place of Presentation: 神戸市
  • Year and Date: 20060410-12
• [Presentation] Too few cells are too much for the plant : organ-wide regulation of leaf size (2006)
  • Author(s): 堀口吾朗
  • Organizer: CDB Symposium 2006理化学研究所発生・再生科学総合研究センター
  • Place of Presentation: 神戸市
  • Year and Date: 20060410-12
• [Book] Orchidaceae. In :"Flora of Mustang" (2008)
  • Author(s): Tsukaya H
  • Total Pages: 471-482
  • Publisher: Kodansha Scientific Ltd.
• [Book] Control of leaf morphogenesis by long-and short-distance signaling : differentiation of leaves into sun or shade types and compensated celle nlargement (2008)
  • Author(s): Fejani, A, Yano, S, Horiguchi, G. and Tsukaya, H
  • Total Pages: 47-62
  • Publisher: PlantCell Monograph series
• [Book] A new member of the CtBP/BARS family from plants : Angustifolia. In : CtBP Family Proteins, Chapter 12, G. Chinnadurai(ed.) (2006)
  • Author(s): Tsukaya H
  • Total Pages: 112-118
  • Publisher: Landes Bioscience, Georgetown, Texas, USA
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/bionev2/jp/index-jp.html