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2010 Fiscal Year Final Research Report

Photosynthesis : dynamics and molecular mechanism of light energy conversion apparatus

Research Project

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Project/Area Number 18GS0318
Research Category

Grant-in-Aid for Creative Scientific Research

Allocation TypeSingle-year Grants
Research InstitutionOkayama University

Principal Investigator

TAKAHASHI Yuichiro  岡山大学, 大学院・自然科学研究科, 教授 (50183447)

Co-Investigator(Kenkyū-buntansha) SHIN Kenjin  岡山大学, 大学院・自然科学研究科, 教授 (60261161)
MINAGAWA Jyun  北海道大学, 低温科学研究所, 准教授 (80280725)
SAKAMOTO Wataru  岡山大学, 資源植物科学研究所, 教授 (20222002)
KASHINO Yasuhiro  兵庫県立大学, 生命理学研究科, 准教授 (20221872)
Project Period (FY) 2006 – 2010
Keywords光合成 / 光化学系 / アンテナ / ダイナミクス / タンパク質分析
Research Abstract

We purified the reaction center complexes involved in oxygenic photosynthesis and determined their subunits and cofactors, and crystalized them to determine the three-dimensional structures. We also examined the molecular mechanism by which the reaction center complexes are assembled from the constituent subunits and cofactors and proposed a working model for the assembly. Furthermore, we revealed the molecular mechanism by which the structure and function of the reaction center complexes are remodeled under varying light environments.

  • Research Products

    (32 results)

All 2012 2011 2010 2009 2008 2007 2006

All Journal Article (31 results) (of which Peer Reviewed: 31 results) Book (1 results)

  • [Journal Article] 5'-Monohydroxyphylloquinone is the dominant naphthoquinone of PSI in the green alga Chlamydomonas reinhardtii2012

    • Author(s)
      Ozawa, S., Kosugi, M., Kashino, Y., Sugimura, T., and Takahashi, Y.
    • Journal Title

      Plant Cell Physiol.

      Volume: 53 Pages: 237-243

    • Peer Reviewed
  • [Journal Article] Psb30 is a photosystem II reaction center subunit and is required for optimal growth in high light in Chlamydomonas reinhardtii.2011

    • Author(s)
      Inoue-Kashino, N., Kashino, Y. and Takahashi, Y.
    • Journal Title

      J. Photochem. Photobiol.

      Volume: 104 Pages: 220-228

    • Peer Reviewed
  • [Journal Article] Isolation of the elusive supercomplexdriving cyclic electron transfer inphotosynthesis.2010

    • Author(s)
      Iwai, M., Takizawa, K., Tokutsu, R., Okamuro, A., Takahashi, Y., Minagawa, J.
    • Journal Title

      Nature

      Volume: 464 Pages: 1210-1213

    • Peer Reviewed
  • [Journal Article] The reduction state of plastoquinoneis a major modulator of in vivochlorophyll fluorescence inChlamydomonas reinhardtii.2010

    • Author(s)
      Hohmann-Marriott, M. F., Takizawa, K., Eaton-Rye, J., Mets, L., and Minagawa, J.
    • Journal Title

      FEBS lett.

      Volume: 584 Pages: 1021-1026

    • Peer Reviewed
  • [Journal Article] Live cell imaging ofphotosystem II antenna dissociation during state transitions.2010

    • Author(s)
      Iwai, M., Yokono, M., Inada, N., Minagawa, J.
    • Journal Title

      Proc. Natl. Acad. Sci. USA.

      Volume: 107 Pages: 2337-2342

    • Peer Reviewed
  • [Journal Article] Structural and functional studies on Ycf12(Psb30) and PsbZ deletion mutants from a thermophilic cyanobacterium.2010

    • Author(s)
      Takasaka K., Iwai M., Umena Y., Kawakami K., Ohmori Y., Ikeuchi M., Takahashi Y., Kamiya N., Shen J.-R.
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1797 Pages: 278-284

    • Peer Reviewed
  • [Journal Article] Photosystem II complex in vivois a monomer.2009

    • Author(s)
      T. Takahashi, N. Inoue-Kashino, S. Ozawa, Y. Takahashi, Y. Kashino, and K. Satoh
    • Journal Title

      J. Biol. Chem.

      Volume: 284 Pages: 15598-15606

    • Peer Reviewed
  • [Journal Article] Biochemical and structural studies of the large Ycf4-Photosystem I assembly complex of the green algaChlamydomonas reinhardtii.2009

    • Author(s)
      S. Ozawa, J. Nield, A. Terao, E. J. Stauber, M. Hippler, H. Koike, J.-D. Rochaix and Y. Takahashi
    • Journal Title

      The Plant Cell

      Volume: 21 Pages: 2424-2442

    • Peer Reviewed
  • [Journal Article] Effects of Site-directed Mutations in the Chloroplast-encoded ycf4 Gene on Photosystem I Complex Assembly in the Green Alga Chlamydomonas reinhardtii.2009

    • Author(s)
      T. Onishi and Y. Takahashi.
    • Journal Title

      Plant and Cell Physiology

      Volume: 50 Pages: 1750-1760

    • Peer Reviewed
  • [Journal Article] Towards structural elucidation of eukaryotic photosystem II : Purification, crystallization and preliminary X-ray diffraction analysis of photosystem II from a red alga2009

    • Author(s)
      Adachi H., Umena Y., Enami I., Henmi, T. Kamiya N., Shen J.-R.
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1787 Pages: 121-128

    • Peer Reviewed
  • [Journal Article] Probing tyrosineZ oxidation in photosystem II core complex isolated from spinach by EPR at liquid helium temperatures2009

    • Author(s)
      Ren Y.-N., Zhang C.-X., Bao H., ShenJ.-R., Zhao J.-Q.
    • Journal Title

      Photosynthesis Research

      Volume: 99 Pages: 127-138

    • Peer Reviewed
  • [Journal Article] Location of chlorideand its possible functions in oxygen-evolving photosystem II revealed by X-ray crystallography.2009

    • Author(s)
      Kawakami K., Umena Y., Kamiya N., Shen J.-R.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 106 Pages: 8567-8572

    • Peer Reviewed
  • [Journal Article] The variegated mutants lacking chloroplastic FtsHs are defective in D1 degradation and accumulate reactive oxygen species.2009

    • Author(s)
      Kato, Y., Miura, E., Ido, K., Ifuku K., and Sakamoto W.
    • Journal Title

      Plant Physiol.

      Volume: 151 Pages: 1790-1801

    • Peer Reviewed
  • [Journal Article] Arrested differentiation of proplastids into chloroplasts in variegated leaves characterized by plastid ultrastructureand nucleoid morphologies2009

    • Author(s)
      Sakamoto, W., Uno, Y., Zhang, Q., Miura, E., Kato, Y., and Sodmergen
    • Journal Title

      Plant Cell Physiol.

      Volume: 50 Pages: 2069-2083

    • Peer Reviewed
  • [Journal Article] The lack of mitochondrial AtFtsH4 protease alters Arabidopsis leaf morphology at the latestage of rosette development under short day photoperiod2009

    • Author(s)
      Gibala, M., Kicia, M., Sakamoto, W., Gola, E., Kubrakiewicz, J., Smakowska, E., and Janska, H.
    • Journal Title

      Plant J.

      Volume: 59 Pages: 685-699

    • Peer Reviewed
  • [Journal Article] Activation of the heterotrimeric G protein a-subunit GPA1 suppresses the ftsh-mediated inhibition of chloroplast development in Arabidopsis2009

    • Author(s)
      Zhang, L., Wei, Q., Wu, W., Cheng, Y., Hu, G., Sun, Y., Zhu, Y., Sakamoto, W., and Huang, J.
    • Journal Title

      Plant J.

      Volume: 58 Pages: 1041-1053

    • Peer Reviewed
  • [Journal Article] Visualization of plastids in pollen grains : involvement of FtsZ1 in pollenplastid division.2009

    • Author(s)
      Tang, L. Y., Nagata, N., Matsushima, R., Chen, Y., Yoshioka, Y., and Sakamoto, W.
    • Journal Title

      Plant Cell Physiol.

      Volume: 50 Pages: 904-908

    • Peer Reviewed
  • [Journal Article] Protein quality control in chloroplasts : a current model of D1 protein degradation in the Photosystem II repair cycle.2009

    • Author(s)
      Kato, Y. and Sakamoto, W.
    • Journal Title

      J. Biochem.

      Volume: 146 Pages: 463-469

    • Peer Reviewed
  • [Journal Article] Evidence for a stable association of Psb30(Ycf12) with photosystem II corecomplex in the cyanobacterium Synechocystis sp. PCC 6803. N.2008

    • Author(s)
      Inoue-Kashino, T. Takahashi, A. Ban, M. Sugiura, Y. Takahashi, K. Satoh and Y. Kashino
    • Journal Title

      Photosynthesis Res.

      Volume: 98 Pages: 323-335

    • Peer Reviewed
  • [Journal Article] Chloroplast biogenesis during rehydration of the resurrection plant Xerophyta humilis : Parallels to theetioplast-chloroplast transition2008

    • Author(s)
      R. A. Ingle, H. Collett, K. Cooper, Y. Takahashi, J. M. Farrant and N. Illing
    • Journal Title

      Plant, Cell and Environment

      Volume: 38 Pages: 1813-1824

    • Peer Reviewed
  • [Journal Article] Molecular Remodeling of Photosystem II during State Transitions in Chlamydomonas reinhardtii.2008

    • Author(s)
      M. Iwai, Y. Takahashi and J. Minagawa
    • Journal Title

      The Plant Cell

      Volume: 20 Pages: 2177-2189

    • Peer Reviewed
  • [Journal Article] Chloroplast-encoded PsbT is required for efficient biogenesis of photosystem II complex in the green alga Chlamydomonas reinhardtii2008

    • Author(s)
      N. Ohnishi and Y. Takahashi
    • Journal Title

      Photosynthesis Res.

      Volume: 95 Pages: 315-322

    • Peer Reviewed
  • [Journal Article] Chloroplast-encoded polypeptide PsbT is involved in the repair of primary electron acceptor QA of photosystem II2007

    • Author(s)
      N. Ohnishi, Y. Kashino, K. Satoh S. Ozawa and Y. Takahashi
    • Journal Title

      Journal of Biological Chemistry

      Volume: 282 Pages: 7107-7115

    • Peer Reviewed
  • [Journal Article] Distinct physiological responses to a high light and low CO2 environment revealed by fluorescence quenching inphotoautotro-phically grown Chlamydomonas reinhardtii2007

    • Author(s)
      Iwai, M., Kato, N., Minagawa, J.
    • Journal Title

      Photosynth. Res.

      Volume: 94 Pages: 307-314

    • Peer Reviewed
  • [Journal Article] The Chlamydomonas genome reveals the evolution of key animal and plant functions.2007

    • Author(s)
      Merchant, S., Grossman, A., et al.
    • Journal Title

      Science

      Volume: 318 Pages: 245-250

    • Peer Reviewed
  • [Journal Article] Aromatic structure of Tyrosine-92 in theextrinsic PsbU protein of red algal Photosystem II is important for its functioning2007

    • Author(s)
      Okumura A., Sano M., Suzuki T., Tanaka H., Nagao R., Nakazato K., Iwai M., Adachi H., Shen J.-R. and Enami I
    • Journal Title

      FEBS Lett.

      Volume: 581 Pages: 5225-5258

    • Peer Reviewed
  • [Journal Article] Chemically induced expression of riceOSB2 under the control of the OsPR1. 1 promoter confers increased anthocyanin accumulation in transgenic rice.2007

    • Author(s)
      Kawahigashi, H., Hirose, S., Iwai, T., Ohashi Y., Sakamoto, W., Maekawa, M., and Ohkawa, Y.
    • Peer Reviewed
  • [Journal Article] Real-Time monitoring of chloroplast gene expression by a luciferase reporter : Evidence for nuclear regulation of chloroplast2006

    • Author(s)
      T. Matsuo, K. Onai, K. Okamoto, J. Minagawa, M. Ishiura
    • Journal Title

      Mol. Cell. Biol.

      Volume: 26 Pages: 863-870

    • Peer Reviewed
  • [Journal Article] Differences in DNA amount in each mitochondrion in tobacco cultured cell and rice root2006

    • Author(s)
      Takanashi, H., Arimura, S., Sakamoto, W., and Tsutsumi, N.
    • Journal Title

      Genes Genet. Syst.

      Pages: 599-621

    • Peer Reviewed
  • [Journal Article] g-Anisotropy of the S2-state manganese cluster in single crystals of cyanobacterial photosystem II studied by W-band electron paramagnetic resonance spectroscopy2006

    • Author(s)
      Matsuoka H., Furukawa K., Kato T., Mino H., Shen J.-R., and Kawamori A
    • Journal Title

      J Phys Chem B

      Volume: 100 Pages: 599-621

    • Peer Reviewed
  • [Journal Article] Quality control of Photosystem II : cleavage of reaction center D1 protein inspinach thylakoids by FtsH protease under moderate heat stress2006

    • Author(s)
      M. Yoshioka, S. Uchida, H. Mori, K. Komayama, S. Ohira, N. Morita, T. Nakanishi and Y. Yamamoto
    • Journal Title

      J Biol. Chem.

      Volume: 281 Pages: 21660-21669

    • Peer Reviewed
  • [Book] J. Light-harvesting proteins. Chlamydomonas sourcebook ed. By David B. Stern2008

    • Author(s)
      Minagawa
    • Publisher
      Academic Press

URL: 

Published: 2013-07-31  

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