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2010 Fiscal Year Final Research Report

Ontogeny and phylogeny of head development in vertebrates

Research Project

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Project/Area Number 18GS0320
Research Category

Grant-in-Aid for Creative Scientific Research

Allocation TypeSingle-year Grants
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

AIZAWA Shinichi  The Institute of Physical and Chemical Research, ボディプラン研究グループ, グループディレクター (60073011)

Co-Investigator(Kenkyū-buntansha) SHIMAMURA Kenji  熊本大学, 発生医学研究所, 教授 (70301140)
HIRATA Tatsumi  国立遺伝学研究所, 総合遺伝研究系, 准教授 (80260587)
Project Period (FY) 2006 – 2010
Keywords脊椎動物系統発生 / 前後軸形成 / オーガナイザー / 頭部誘導 / 脳形成 / 皮質形成 / 比較発生学 / 比較ゲノム解析
Research Abstract

Comparative analyses of mammalian (mouse, rabbit, pig, suncus), reptile (gekko, turtle), avian (chick), amphibian (Xenopus), actinist (coelacanth), cladist (bichir), teleost (zebrafish, medaka, fugu), cartillaginous fish (skate) and agnatha (lamprey) embryos, developmentally and/or genomically, demonstrated that 1) extraembryonic head organizer tissues in vertebrates are homologous to deep endoderm cells in Xenopus, 2) Otx2 gene cascade was established in ancestral sarcopterigian to suppress poeteriorizing signals, 3) anterior neuroectoderm develops differently between sarcopterigian and actinopterigian, 4) potency to form layer structures in cortical progenitor cells would have been established in reptile stage, not in mammal, and others.

  • Research Products

    (39 results)

All 2011 2010 2009 2008 2007 2006 Other

All Journal Article (24 results) (of which Peer Reviewed: 24 results) Presentation (12 results) Book (2 results) Remarks (1 results)

  • [Journal Article] MicroRNA-9 regulates neurogenesis in mouse telencephalon by targeting multiple transcription factors.2011

    • Author(s)
      Shibata M., Nakao H., Kiyonari H., Abe T., Aizawa S.
    • Journal Title

      J Neurosci. 31

      Pages: 3407-3422

    • Peer Reviewed
  • [Journal Article] Establishment of conditional reporter mouse lines at ROSA26 locus for live cell imaging.2011

    • Author(s)
      Abe T, Kiyonari H, Shioi G, Inoue KI, Nakao K, Aizawa, S, Fujimori T
    • Journal Title

      Genesis. (In print)

    • Peer Reviewed
  • [Journal Article] A mouse reporter line toconditionally mark nuclei and cell membranes for in vivo live-imaging.2011

    • Author(s)
      Shioi G, Kiyonari H, Abe T, Nakao K, Fujimori T, Jang CW, Huang CC, Akiyama H, Behringer RR, Aizawa, S
    • Journal Title

      Genesis. (In print)

    • Peer Reviewed
  • [Journal Article] Evolutionary origin of the Otx2 enhancer for its expression in visceral endoderm.2010

    • Author(s)
      Kurokawa D., Ohmura T., Ogino H., Takeuchi M., Inoue A., Inoue F., Suda Y., Aizawa S.
    • Journal Title

      Dev.Biol. 342

      Pages: 110-120

    • Peer Reviewed
  • [Journal Article] Three inhibitors of FGF receptor, ERK, and GSK3 establishes germline-competent embryonic stem cells of C57BL/6N mouse strain with high efficiency and stability.2010

    • Author(s)
      Kiyonari H., Kaneko M., Abe S., Aizawa S.
    • Journal Title

      Genesis 48

      Pages: 317-327

    • Peer Reviewed
  • [Journal Article] The same enhancer regulates the earliest Emx2 expression in caudal forebrain primordium, subsequent expression in dorsal telencephalon and later expression in the cortical ventricular zone.2010

    • Author(s)
      Suda Y., Kokura K., Kimura J., Kajikawa E., Inoue F., Aizawa S.
    • Journal Title

      Development 137

      Pages: 2939-2949

    • Peer Reviewed
  • [Journal Article] Otx2 and Otx1 protect diencephalon and mesencephalon from caudalization into metencephalon during early brain regionalization.2010

    • Author(s)
      Sakurai Y., Kurokawa D., Kiyonari H., Kajikawa E., Suda Y., Aizawa S.
    • Journal Title

      Dev.Biol. 347

      Pages: 392-403

    • Peer Reviewed
  • [Journal Article] Proteomics Analysis of the Temporal Changes in Axonal Proteins during Maturation.2010

    • Author(s)
      Yamatani H., Kawasaki T., Mita., S., Inagaki N., Hirata T.
    • Journal Title

      Dev.Neurobiol. 70

      Pages: 523-537

    • Peer Reviewed
  • [Journal Article] Evolution of Otx paralogue usages in early patterning of the vertebrate head.2009

    • Author(s)
      Suda Y., Kurokawa D., Takeuchi M., Kajikawa E., Kuratani S., Amemiya C., Aizawa S.
    • Journal Title

      Developmental Biology 325

      Pages: 282-295

    • Peer Reviewed
  • [Journal Article] Sfrp controls apicobasal polarity and oriented cell division in developing gut epithelium.2009

    • Author(s)
      Matsuyama M., Aizawa S., Shimono A.
    • Journal Title

      PLos Genet. 5

      Pages: e1000427

    • Peer Reviewed
  • [Journal Article] Germ layer patterning in bichir and lamprey ; an insight into its evolution in vertebrates.2009

    • Author(s)
      Takeuchi M., Takahashi M., Okabe M., Aizawa S.
    • Journal Title

      Developmental Biology 332

      Pages: 90-102

    • Peer Reviewed
  • [Journal Article] EPB41L5 functions to post-transcriptionally regulate cadherin and integrin during epithelial-mesenchymal transition.2008

    • Author(s)
      Hirano M., Hashimoto S., Yonemura S., Sabe H., Aizawa S.
    • Journal Title

      Journal of Cell Biology 182

      Pages: 1217-1230

    • Peer Reviewed
  • [Journal Article] MicroRNA-9 modulates Cajal-Retzius cell differentiation by suppressing Foxg1 expression in mouse medial pallium.2008

    • Author(s)
      Shibata M., Kurokawa D., Nakao H., Ohmura T., Aizawa S.
    • Journal Title

      J Neurosci. 28

      Pages: 10415-21

    • Peer Reviewed
  • [Journal Article] Retrospective on reverse genetics in mice around the wourld and in Japan.2008

    • Author(s)
      Aizawa S.
    • Journal Title

      Develop.Growth Differ. 50

      Pages: S29-S34

    • Peer Reviewed
  • [Journal Article] Experience-Dependent Transfer of Otx2 Homeoprotein into the visual Cortex Activates Postnatal Plasticity.2008

    • Author(s)
      Sugiyama S., Nardo AD., Aizawa S., Matsuo I., Volovitch M., Prochiantz A., Hensch TK.
    • Journal Title

      Cell 134

      Pages: 208-520

    • Peer Reviewed
  • [Journal Article] Semaphorin 3F confines ventral tangential migration of lateral olfactory tract neurons onto the telencephalon surface.2008

    • Author(s)
      Ito K., Kawasaki T., Takashima S., Matsuda I., Aiba A., Hirata T.
    • Journal Title

      J.Neurosci. 28

      Pages: 4414-4422

    • Peer Reviewed
  • [Journal Article] Method for electroporation for the early chick embryo.2008

    • Author(s)
      Hatakeyama J., Shimamura K.
    • Journal Title

      Develop.Growth Differ. 50

      Pages: 449-452

    • Peer Reviewed
  • [Journal Article] Acetylated YY1 regulates Otx2 expression in anterior neuroectoderm at two cis-sites 90kb apart.2007

    • Author(s)
      Takasaki N., Kurokawa D., Nakayama R., Nakayama J., Aizawa, S.
    • Journal Title

      The EMBO Journal 26

      Pages: 1649-1659

    • Peer Reviewed
  • [Journal Article] Crucial roles of Foxa2 in mouse anterior-posterior axis polarization via regulation of anterior visceral endoderm-specific genes.2007

    • Author(s)
      Kimura-Yoshida C., Tian E., Nakano H., Amazaki S., Shimokawa K., Rossant J., Aizawa S., Matsuo I.
    • Journal Title

      PNAS 104

      Pages: 5919-5924

    • Peer Reviewed
  • [Journal Article] Mouse homologues of Shisa antagonistic to Wnt and Fgf signalings.2007

    • Author(s)
      Furushima K., Yamamoto A., Nagano T., Shibata M., Miyachi H., Abe T., Ohshima N., Kiyonari H., Aizawa S.
    • Journal Title

      Developmental Biology 306

      Pages: 480-492

    • Peer Reviewed
  • [Journal Article] Sfrp1 and Sfrp2 regulate anteroposterior axis elongation and somite segmentation during mouse embryogenesis.2006

    • Author(s)
      Satoh W., Gotoh T., Tsunematsu Y., Aizawa S., Shimono A.
    • Journal Title

      Development 133

      Pages: 989-999

    • Peer Reviewed
  • [Journal Article] A new serine/threonine protein kinase, Omphk1, essential to ventral body wall formation.2006

    • Author(s)
      Hirano M., Kiyonari H., Inoue A., Furushima K., Murata T., Suda Y., Aizawa S.
    • Journal Title

      Developmental Dynamics 235

      Pages: 2229-2237

    • Peer Reviewed
  • [Journal Article] Shisa2 promotes the maturation of somatic precursors and transition to the segmental fate in Xenopus embryos.2006

    • Author(s)
      Nagano T., Takehara S., Takahashi M., Aizawa S., Yamamoto, A.
    • Journal Title

      Development 133

      Pages: 4643-4654

    • Peer Reviewed
  • [Journal Article] Evolutionary constraint on Otx2 neuroectoderm enhacers-deep conservation from skate to mouse and unique divergence in teleost.2006

    • Author(s)
      Kurokawa D., Sakurai Y., Inoue A., Nakayama R., Takasaki N., Suda Y., Miyake T., Amemiya C., Aizawa S.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 103

      Pages: 19350-19355

    • Peer Reviewed
  • [Presentation] MicroRNA-9 regulates neurogenesis in mouse telencephalon by targeting multiple transcription factors2011

    • Author(s)
      Shinichi Aizawa
    • Organizer
      2011 Meeting of German Society of Developmental Biologists
    • Place of Presentation
      Dresden, Germany
    • Year and Date
      20110323-20110326
  • [Presentation] The adherens junction serves as a switch for neurogenesis via Notch signaling2010

    • Author(s)
      Kenji Shimamura
    • Organizer
      Notch and Stem Cells
    • Place of Presentation
      Athens, Greece
    • Year and Date
      20101003-20101006
  • [Presentation] Otx1 and Otx2 functions in forebrain and midbrain development.2010

    • Author(s)
      Shinichi Aizawa
    • Organizer
      Society for Developmental Biology 69th annual meeting
    • Place of Presentation
      New Mexico, USA
    • Year and Date
      20100805-20100809
  • [Presentation] Conservation of developmental mechanisms in evolutionarily divergent brain structures.2010

    • Author(s)
      Tatsumi Hirata
    • Organizer
      SYMPOSIA PARIS meeting 2010, Euro Evo Devo
    • Place of Presentation
      Paris, France
    • Year and Date
      20100706-20100709
  • [Presentation] The same enhancer regulates the earliest Emx2 expression in caudal forebrain primordium, subsequent expression in dorsal telencephalon and later expression in cortical ventricular zone.2009

    • Author(s)
      Shinichi Aizawa
    • Organizer
      16th International Society of Developmental Biologists Congress.
    • Place of Presentation
      Edinburgh, United Kingdom
    • Year and Date
      20090906-20090910
  • [Presentation] Role of the adherens junction in neurogenesis2009

    • Author(s)
      Kenji Shimamura
    • Organizer
      16^<th> international Society of Developmental Biologists Congress 2009
    • Place of Presentation
      Edinburgh, UK
    • Year and Date
      20090906-20090910
  • [Presentation] Emergence of the layer structure in the mammalian neocortex associated with a change in the stem cells dynamics2009

    • Author(s)
      Tatsumi Hirata
    • Organizer
      Cold Spriing Harbor Laboratory SYMPOSIUM LXXIV, Symoposium on Evolution
    • Place of Presentation
      Cold Spring Harbor, NY
    • Year and Date
      2009-05-27
  • [Presentation] Axon outgrowth inhibition mediated by four-transmembrane protein M6a.2008

    • Author(s)
      Tatsumi Hirata
    • Organizer
      Japan/USA Joint Meeting on Vertebrate Organogenesis
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      20081123-20081124
  • [Presentation] Otx2 and A-P axis formation in vertebrates2008

    • Author(s)
      Shinichi Aizawa
    • Organizer
      Japan/USA Joint Meeting on Vertebrate Organogenesis
    • Place of Presentation
      Houston, USA
    • Year and Date
      20081120-20081121
  • [Presentation] The adherens junction is required for the regulation of neural differentiation2008

    • Author(s)
      Kenji Shimamura
    • Organizer
      Frontiers in Developmental Biology
    • Place of Presentation
      Giens Peninsula, France
    • Year and Date
      20080913-20080917
  • [Presentation] Evolutionary constraint on Otx2-neuroectoderm enhancers ; deep conservation from skate to mouse and unique divergence in teleost.2007

    • Author(s)
      Shinichi Aizawa
    • Organizer
      FIRST PAN AMERICAN CONGRESS IN DEVELOPMENTAL BIOLOGY
    • Place of Presentation
      Cancun, Mexico
    • Year and Date
      20070616-20070620
  • [Presentation] Otx2 gene cascade in A-P axis formation2006

    • Author(s)
      Shinichi Aizawa
    • Organizer
      Monterotondo Mouse Biology Meeting
    • Place of Presentation
      Monterotondo, Italy
    • Year and Date
      20060419-20060420
  • [Book] In Emerging Model Organisms(The Genus Polypterus (Bichirs) ; A fish group diverged at the stem of Ray-finned fishes (Actinopterygii) volume 1)2009

    • Author(s)
      Takeuchi M., Okabe M., Aizawa S.
    • Total Pages
      447-468
    • Publisher
      Cold Spring Harbor Laboratory Press
  • [Book] Electroporation and Sonoporation in Dvelopmental Biology.(Method of Electroporation for the Early Chick Embryo.)2009

    • Author(s)
      Hatakeyama J., Shimamura K.
    • Total Pages
      43-54
    • Publisher
      Springer
  • [Remarks] ホームページ

    • URL

      http://www.cdb.riken.go.jp/vbp/

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Published: 2012-01-26   Modified: 2015-07-23  

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