2020 Fiscal Year Final Research Report

Two-dimensional super-thin layer chromatography / nano-structure assisted laser desorption ionization mass spectrometry using nanowire device

Research Project

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Project/Area Number	18H02009
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 34020:Analytical chemistry-related
Research Institution	Tokyo University of Pharmacy and Life Science
Principal Investigator	Umemura Tomonari 東京薬科大学, 生命科学部, 教授 (10312901)
Co-Investigator(Kenkyū-buntansha)	安井隆雄名古屋大学, 工学研究科, 准教授 (00630584) 中嶋秀東京都立大学, 都市環境科学研究科, 准教授 (10432858) 手嶋紀雄愛知工業大学, 工学部, 教授 (30292501)
Project Period (FY)	2018-04-01 – 2021-03-31
Keywords	ナノワイヤ / 酸化亜鉛 / 薄層クロマトグラフィー / レーザー脱離イオン化 / 質量分析 / 脂質
Outline of Final Research Achievements	Although lipidomics, the study of lipids, have a great potential as a clinical tool for monitoring metabolic changes in health and disease, the analytical techniques for lipids have not made substantial advances during the last decade. In order to promote lipidomics research, in the present project, we have undertaken the development of zinc oxide nanowire array-grown glass substrate plates, which have a dual role as a thin-layer chromatography (TLC) plate and a target plate for laser desorption/ionization (LDI) . We have refined hydrothermal synthesis technique and succeeded in producing a variety of nanowires of an intended size on a glass substrate. We have also developed an interface device for putting the UTLC plate into commercially available conventional MALDI-MS instrument.
Free Research Field	分離分析
Academic Significance and Societal Importance of the Research Achievements	科学の進歩には革新的な分析法・分析技術の開発が不可欠である。本研究は、1細胞での分離分析を実現しうるポテンシャルのある分析法として学術的な意義は大きいと思われる。