2020 Fiscal Year Final Research Report

Development of photoresponsive DNA nanostructure to accerelate iRed based nucleic acid medicine

Research Project

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Project/Area Number	18H02108
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 37030:Chemical biology-related
Research Institution	The University of Tokushima
Principal Investigator	MINAKAWA Noriaki 徳島大学, 大学院医歯薬学研究部(薬学域), 教授 (40209820)
Co-Investigator(Kenkyū-buntansha)	小暮健太朗徳島大学, 大学院医歯薬学研究部(薬学域), 教授 (70262540) 田良島典子徳島大学, 大学院医歯薬学研究部(薬学域), 講師 (90755183) 山本清義千葉県がんセンター(研究所), がん治療開発グループがん遺伝創薬研究室, 研究員 (80783521)
Project Period (FY)	2018-04-01 – 2021-03-31
Keywords	核酸創薬 / RNAi医薬 / iRed / 核酸ナノ構造体 / 微弱電流
Outline of Final Research Achievements	In this study, we examined the construction of iRed-loaded nucleic acid nanostructures and confirmed the formation of square-shaped nucleic acid nanostructures. Although we have not found a condition for all of the molecules to converge on this structure, the fact that we were able to construct nucleic acid nanostructures from complementary double-stranded DNA is highly evaluated in the field of nanochemistry. We also succeeded in constructing a system for releasing nucleic acid drug molecules by photoirradiation by using triangular prismatic nucleic acid nanostructures. We have demonstrated the possibility of solving the problem of DDS, which is a bottleneck in the development of nucleic acid drug molecules, by combining this technology with the intracellular delivery by fET.
Free Research Field	核酸創薬化学
Academic Significance and Societal Importance of the Research Achievements	現在、次世代型の医薬分子として核酸を利用する核酸医薬品が高い注目をあびている。しかし核酸医薬品開発研究においては、極性高分子の核酸をどのように細胞内に送達させるかが大きな問題点となっている。本研究課題では、研究代表者らが開発した核酸医薬分子intelligent RNA expression device (iRed) をナノ構造体に搭載し、微弱電流で印加することで問題点の解決を目指した。この研究成果により核酸医薬品開発が加速することが期待される。