Molecular mechanism on PRR (Pseudo Response Regulators)-mediated epigenetic regulation underlying robust circadian clock oscillation in higher plants

2022 Fiscal Year Final Research Report

• Project/Area Number: 18H02137
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 38030:Applied biochemistry-related
• Research Institution: Nagoya University
• Principal Investigator: Yamashino Takafumi 名古屋大学, 生命農学研究科, 准教授 (00314005)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Keywords: 植物の概日時計 / 転写調節 / エピゲノム

Outline of Final Research Achievements

The gate effect of the biological clock supports oscillations of clock genes that are robust to environmental fluctuations by restricting the input of environmental signals to specific time periods. DNA methylation and histone acetylation in the regulatory regions of gene expression are known to cause conformational changes in chromatin that define the potential for transcriptional activity. In this study, we focus on the PRR family and its target gene CCA1/LHY, which form a negative feedback loop circuit in the central oscillator of Arabidopsis thaliana, to clarify how the PRR family plays a role in the regulation of chromatin structure in the central oscillator.

Free Research Field

植物生理学

Academic Significance and Societal Importance of the Research Achievements

概日リズムを生成する植物時計の中心振動体タンパク質は外環境に同調しながら24時間周期で発現レベルが変動することが知られている。この振動の振幅は100倍以上の振れ幅をもち自由継続する性質も兼ね備えている。本研究では中心振動体のrobustな振動特性を支える背景にはエピゲノム制御を介した日周レベルのクロマチンダイナミズムが存在することを明らかにした。