2020 Fiscal Year Annual Research Report
Cell type-specific mechanisms of desiccation tolerance in the anhydrobiotic insect
| Project/Area Number |
18H02217
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
グセフ オレグ 国立研究開発法人理化学研究所, 科技ハブ産連本部, ユニットリーダー (30711999)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | anhydrobiosis / CAGEseq / nervous system / scRNAseq / desiccation tolerance / glia cells |
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| Outline of Annual Research Achievements |
The final year of the project logically wrapped up the whole research program. Using combination of bulk CAGE-Seq data and pilot single cell transcriptomics data we confirmed that there is clear tissue-specific and cell-type specific genetic program associated with complete desiccation tolerance in an anhydrobiotic insect. For example, using advanced bioinformatics approach we have discovered a special cell population in Pv11 cell culture that express unique profile of anhydrobiosis-associated genes (LIL proteins, PIMT, etc.). Before desiccation, this sub-population has occupied only 17% of whole population. After the re-hydration, this cell population occupied the majority of the cells, and, thus, we concluded that overall 15-20% survival of Pv11 is directly linked to the special transcriptional profile. At the same time we are not exactly sure what kind of mechanism trigger the successful genetic signature is not known and we will analyze it in a future studies. We also made sufficient progress in analyzing nervous system of the anhydrobiotic chironomid. We found that there is a specific set of protective genes exclusively active in brains of the insects. Our pilot data on single cell analysis further showed that cell types in brain have unique transcriptional profile upon desiccation. For example, on the current step we successfully identified the glia cells and characterized "effective gene set" providing protection against desiccation in this cell type. Finally we launched MidgeBase 3.0 database, with detail gene expression data based on the results of the project.
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| Research Progress Status |
令和2年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和2年度が最終年度であるため、記入しない。
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Research Products
(5 results)
