Establishment of a novel CAR-T therapy for canine melanoma

2020 Fiscal Year Final Research Report

• Project/Area Number: 18H02347
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Yamaguchi University
• Principal Investigator: Mizuno Takuya 山口大学, 共同獣医学部, 教授 (90398826)
• Co-Investigator(Kenkyū-buntansha): 野口 俊助 大阪府立大学, 生命環境科学研究科, 准教授 (10701295) ; 今村 拓也 広島大学, 統合生命科学研究科(理), 教授 (90390682)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 犬 / がん / CAR-T / 免疫細胞療法

Outline of Final Research Achievements

Canine oral melanoma is a highly malignant tumor for which no effective systemic therapy has been established. The purpose of this study was to establish an effective immune cell therapy (CAR-T cell therapy) for oral melanoma and to establish a basic technology for CAR-T cell therapy in dogs by examining detailed conditions such as efficiency and effectiveness of CAR-T cell therapy in dogs.
Unfortunately, we were not able to produce a good CAR-T specific for oral melanoma based on the gene expression profile we obtained, but we were able to examine the conditions for the production of canine CAR-T cells and prove their efficacy in mice.

Free Research Field

獣医内科学

Academic Significance and Societal Importance of the Research Achievements

本研究で得られた成果は、犬の悪性腫瘍に対する免疫細胞療法としてCAR-T細胞を用いる上での基礎となる情報として非常に重要である。犬のCAR-T細胞療法は、まだ未開拓分野であり、世界的にも着手しているグループは少ない。今回得られた情報をもとにさらに改良をすることにより、犬の悪性腫瘍に対するCAR-T細胞療法が臨床の現場で使用できるようになり、新たな治療オプションとなる可能性がある。