2020 Fiscal Year Final Research Report
A novel super-resolution imaging method for capturing individual molecular dynamics in cardiac myosin ensembles
| Project/Area Number |
18H02408
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 43040:Biophysics-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Kaya Motoshi 東京大学, 大学院理学系研究科(理学部), 助教 (00422098)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | 心筋ミオシン / 心臓収縮 / 超解像イメージング |
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| Outline of Final Research Achievements |
In general, when the distance between two molecules is less than 200 nm, the position of each molecule cannot be detected due to the limitation of the spatial resolution of the microscope. Therefore, a super-resolution imaging methods have been developed. However, the conventional methods have low temporal resolution and thus, we attempted to achieve super-resolution imaging with microsecond resolution, which is sufficient to see the fluctuations of proteins. As a result, we succeeded in capturing the interaction of two myosin molecules with actin at a distance of 150 nm with a time resolution of 50 microseconds. In addition, we found a unique structural change in cardiac myosin, which allows cardiac myosin ensembles to execute synchronous force generation, resulting in efficient heart contraction.
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| Free Research Field |
生物物理
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| Academic Significance and Societal Importance of the Research Achievements |
タンパク質の動態を捉えることのできる時間分解能を持つ超解像顕微鏡は現状存在しないが，本研究では，十分な時間分解能を持つ超解像イメージング法の礎となる成果を得ることができた．本手法を更に発展させれば，細胞内の各分子の詳細な動態を追従できる画期的な超解像イメージング法が確立されることが期待される．また，心筋ミオシンの研究成果は，心筋症の発症機構や新薬の開発において大いに役立つと期待できる．
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