2020 Fiscal Year Annual Research Report
A next-generation mass spectrometric/computational strategy for aging biomarker discovery
| Project/Area Number |
18H02425
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Wu Yibo 国立研究開発法人理化学研究所, 生命医科学研究センター, 上級研究員 (50811618)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | Proteomics / Obesity / Adipose tissue cells / Ligand-receptor pairs / Autocrine |
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| Outline of Annual Research Achievements |
In FY2019, we have generated time-course cell type-specific proteomics data for adipose tissue cells comparing high-fat and control diet fed mice, and have found a few candidate genes. In FY2020, we focused on validating function of the chloride intracellular channel protein 4 (Clic4) in macrophages. Interestingly, in apoptotic adipocyte (apoAP)-treated RAW 264.7 cells, we observed increased lipid accumulation upon Clic4 knockdown. We then performed proteomic and lipidomic analyses and found enhanced OXPHOS in Clic4-deficient macrophages. Besides, we expanded the analysis to study ligand-receptor pairs for both intercellular communication and autocrine of the 5 analyzed cell types. We found ligand-receptor pairs that were significantly regulated by diet in a time-dependent manner.
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| Research Progress Status |
令和2年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和2年度が最終年度であるため、記入しない。
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| Remarks |
The PreadProfiler is a comprehensive and integrated dataset developed by the Wu Lab and Gupta Lab that provides the transcriptomic and proteomic profiles of murine PDGFRb+ adipose tissue progenitors.
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