2018 Fiscal Year Annual Research Report
The counterbalancing forces underlying polarity-coupled epithelial cell height control prior to and during epithelial folding
| Project/Area Number |
18H02441
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
WANG YUCHIUN 国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (80725995)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | microtubule forces / CAMSAP/Patronin / epithelial cell shape |
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| Outline of Annual Research Achievements |
In the FY 2018, Our research efforts revolve around the following three areas:1) We have been seeking to improve our imaging methods to better characterize the apical dome dynamics, including cell shape, microtubules, Patronin and other proteins that may be involved. 2) We have also been working on procedures of image processing, quantitation, 3D visualization and analysis and have established a few analysis pipelines using a combination of FIJI, 3DSlicer, and custom made Python code. 4) We have begun designing strategies and constructing fly lines that could allow us to achieve these goals. 5) In addition, we have been seeking to develop optogenetic methods for temporal-spatially specific perturbation of apical dome mechanics. These will require testing of a large number of construct designs. We have started and will continue these efforts in the next fiscal year. 6) Furthermore, we have began working with Dr. Tatsuo Shibata on numerical simulation of apical dome mechanics based on a modeling framework for the dynamics behaviors of a disordered filamentous network that contains cross-linkers and motor proteins.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Although in the past physical year, we have not been able to obtain any concrete results, this was expected at the outset as we remained in the methodology development phase. Once the critical technical improvements are achieved, we would expect to advance the project much more rapidly.
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| Strategy for Future Research Activity |
In the FY 2019, we will will continue what was left off from the previous physical year. We hope to complete our methodology development phase and begin to acquire new data. We will work the following aspects of this project.1) Assessment of phenotypes with perturbation of the Dynein motor, the candidate apical anchorage proteins, Shot and Spectrin, in terms of Patronin dynamics, microtubule structure and cell and tissue morphology.2) Begin to investigate the actin structure and its relation with the microtubule network, the apical anchorage proteins, and adherens junctions. 3) Develop ontogenetic tools for the manipulation of Patronin, Katanin and the candidate apical dome proteins.
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