2019 Fiscal Year Annual Research Report
Molecular regulation of mesothelial EMT in avian chorioallantoic fusion
| Project/Area Number |
18H02452
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| Research Institution | Kumamoto University |
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Principal Investigator |
Sheng Guojun 熊本大学, 国際先端医学研究機構, 特別招聘教授 (20399439)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | mesothelium / chicken / chorioallantois / EMT |
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| Outline of Annual Research Achievements |
In the second year of this project (2019 fiscal year), we have achieved major parts of aims 2-4. More specifically, we have uncovered involvement of canonical EMT regulatory gene (SNAI1) and non-canonical EMT regulatory gene (WT1) during CAM fusion. We have performed gene expression analysis of those transcription factors. We have also made CRISPR mediated gene activation (CRISPR-a) and inhibition (CRISPR-i) constructs for these two genes and performed gain- and loss-of function studies in CAM culture ex ovo. We have optimized ex vivo CAM culture and DNA construct electroporation methodology. We have performed immunofluorescence studies of CAM samples for EMT marker gene expression and have performed electron microscopy studies of fusing CAM tissues for cell biological studies.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The project is making progress as we have originally planned.
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| Strategy for Future Research Activity |
The final year of this project, we will clarify the difference between allantoic mesothelial EMT and chorionic mesothelial EMT. We will complete the functional study of WT1 and SNAI1 in mesothelial EMT fusion. We will also complete the EM morphological study of CAM fusion. We will summarize our data and prepare manuscript for submission in the 2020 fiscal year.
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Research Products
(3 results)
