Understanding temporal coding of stress signaling specificity

2020 Fiscal Year Final Research Report

• Project/Area Number: 18H02483
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 45010:Genetics-related
• Research Institution: Hiroshima University (2020); Shiga University of Medical Science (2018-2019)
• Principal Investigator: Takuma Sugi 広島大学, 統合生命科学研究科(理), 特任准教授 (70571305)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: C. elegans / stress signaling

Outline of Final Research Achievements

In this study, using the nematode C. elegans as a model animal, we attempted to elucidate the mechanism of how diverse environmental stresses processed via specific molecules to exert different behavioral responses. Since the biochemical analysis in the preliminary experiments revealed the possibility that the activity of MAP kinase oscillates over time, we attempted to analyze the function of this molecule in vivo. We succeeded in developing a system to measure the activity. On the other hand, we did not detect any change in the activity of the molecule when we targeted only AVA as a specific neuron. Therefore, we are now attempting to detect the oscillation comprehensively using a microscope developed to detect the activity of all neurons simultaneously.

Free Research Field

生物物理学

Academic Significance and Societal Importance of the Research Achievements

本研究から, In vivoで分子活性を検出するための２種類の顕微鏡を開発することに成功し, 論文化されたことは学術的意義として大きいことと考えられる. 一方で, 生体メカニズムとしてはまだ不明瞭な点が残された. 今後, これらのメカニズムの解析から, ストレス応答の特異性を決定する機構について, さらに知見が積み重なることで, 社会的意義も得られると期待される.