2020 Fiscal Year Final Research Report
Absolute quantitative analysis of in vivo oxygen concentration
| Project/Area Number |
18H02614
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 48040:Medical biochemistry-related
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| Research Institution | Doshisha University (2020)
Osaka University (2018-2019) |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | 破骨細胞 / 酸素 / 寿命イメージング / 二光子励起顕微鏡 / りん光 / 時間相関単一光子計数 / イリジウム錯体 / エネルギー代謝 |
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| Outline of Final Research Achievements |
Oxygen is essential for maintaining homeostasis of living organisms through various cell response mechanisms. However, the oxygen concentration to which individual cells are exposed in the living body is ambiguous. Here, by developing and improving the latest bioimaging technology using a multiphoton excitation microscope, we analyzed the oxygen concentration of individual cells in the tissues of living animals at the single cell level. We measured phosphorescence lifetime of osteoclasts in the bone marrow using the phosphorescence lifetime imaging method. As a result, we succeeded in determining the range of physiological oxygen concentration of osteoclasts is 2 to 5%.
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| Free Research Field |
生化学、薬理学、骨代謝学
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| Academic Significance and Societal Importance of the Research Achievements |
酸素の働きを質の観点で解き明かしてきた従来知見に対して、本研究は酸素の量の情報を明らかにした。即ち、生体内で個々の細胞がどの程度の酸素濃度で維持され、それがどのくらい変動するかの定量的知見を明らかにした。本研究成果は、細胞生物学などの基礎研究だけでなく、がんや虚血性疾患などの低酸素状態が関係する様々な病気の基礎病態を定量的に理解するための重要な知見となることが期待される。
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