Analyzing the substrate multispecificities of eukariotic proton-coupled oligopeptide transporters

2020 Fiscal Year Final Research Report

• Project/Area Number: 18H03195
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 59040:Nutrition science and health science-related
• Research Institution: University of Shizuoka
• Principal Investigator: Ito Keisuke 静岡県立大学, 食品栄養科学部, 准教授 (40580460)
• Co-Investigator(Kenkyū-buntansha): 加藤 竜司 名古屋大学, 創薬科学研究科, 准教授 (50377884) ; 田中 瑞己 静岡県立大学, 食品栄養科学部, 助教 (70803344) ; 河原崎 泰昌 静岡県立大学, 食品栄養科学部, 准教授 (80303585)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: ペプチド輸送体 / ジペプチド / 栄養素吸収

Outline of Final Research Achievements

Peptide uptake system is conserved across organisms from bacteria to higher animals and plants and is important for acquiring nitrogen resources with high efficiency. The molecules involved in this system are categorized into the Proton-coupled Oligopeptide Transporter (POT) family. POT can recognize as many as 8,400 types of di/tripeptides. In this study, we analyzed the substrate multispecificities of the eukariotic POTs. Eukariotic POTs were involved in the preferential uptake of essential amino acids in humans, which impose a biosynthesis burden on organisms. Moreover, an in silico POT affinity prediction models using ‘combinations of physicochemical compound properties’ were constructed with reasonable accuracy. Our results provide the POT molecular basis to develop high-absorbable peptides.

Free Research Field

食品化学

Academic Significance and Societal Importance of the Research Achievements

本研究によって得られた成果は、食品ペプチドの吸収実態の理解を通じ、病中病後の経腸栄養療法への応用、スポーツ用途食品の開発、高齢者や発展途上国における高効率な栄養供給法の開発等につながる。また、β-ラクタム系抗生物質、抗がん剤、降圧薬、抗ウィルス薬等のペプチド類似構造を持つ医薬品がPOTを介して吸収されることが報告されていることから、医薬品開発や食薬相互作用の理解への貢献も期待できる。