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2020 Fiscal Year Annual Research Report

A Sustainable and Recycling-oriented Wastewater Industry by Maximizing Valuable Resources and Energy Recovery Using the Novel Algal-bacterial Aerobic Granular Sludge Technology

Research Project

Project/Area Number 18H03403
Research InstitutionUniversity of Tsukuba

Principal Investigator

雷 中方  筑波大学, 生命環境系, 准教授 (30634505)

Co-Investigator(Kenkyū-buntansha) 張 振亜  筑波大学, 生命環境系, 教授 (20272156)
清水 和哉  筑波大学, 国際室, 准教授 (10581613)
水野谷 剛  筑波大学, 生命環境系, 准教授 (10500770)
Project Period (FY) 2018-04-01 – 2022-03-31
KeywordsAlgal-bacterial AGS; / Bacterial AGS; / Microalgae; / Energy gain; / Resource conservation; / Salinity stress
Outline of Annual Research Achievements

During year 2020, the followings have been confirmed.
(1) Granulation can be successfully achieved from single cell microalgae by using the similar operation strategy as for naturally growing algae and for activated sludge.
(2) Algal-bacterial aerobic granular sludge (AGS) system has higher and stable potentials for P removal and recovery from wastewater with low energy/carbon source consumption and less influence by salinity due to the co-existence of algae.
(3) Algal-bacterial AGS also possesses higher potential for Cr biosorption and recovery from wastewater under neutral condition, mainly attributable to its effective bioreduction capability, intracellular accumulation and algae participation.
(4) The profits by using this novel system have been estimated in the context of a local wastewater treatment plant.

Current Status of Research Progress
Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

We have finished most of the designed experiments. Based on the fruitful results from this project during the past three years, we are planning to extend our focus to a new research direction of this novel wastewater treatment system this year.

Strategy for Future Research Activity

(1) to further confirm the resources (especially phosphorus and alginate-like exopolysaccharides) and energy recovery potentials from the continuous operation of the algal-bacterial AGS system.
(2) to compare the differences in energy/resources recovery and greenhouse gases emission reduction between the bacterial AGS and algal-bacterial AGS systems; and
(3) to establish the preliminary model for the algal-bacterial AGS system in the context of a local wastewater treatment plant (WWTP), i.e., Shimodate WWTP in Ibaraki Prefecture.

  • Research Products

    (37 results)

All 2021 2020 Other

All Journal Article (15 results) (of which Int'l Joint Research: 10 results,  Peer Reviewed: 15 results) Presentation (18 results) (of which Int'l Joint Research: 18 results,  Invited: 1 results) Remarks (4 results)

  • [Journal Article] Recent advancements in nanobubble water technology and its application in energy recovery from organic solid wastes towards a greater environmental friendliness of anaerobic digestion system2021

    • Author(s)
      Wang X., Lei Z.*, Shimizu K., Zhang Z., Lee D.-J.
    • Journal Title

      Renewable and Sustainable Energy Reviews

      Volume: 145 Pages: 111074

    • DOI

      10.1016/j.rser.2021.111074

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Performance and stability of biogas recirculation-driven anaerobic digestion system coupling with alkali addition strategy for sewage sludge treatment2021

    • Author(s)
      Zhao J., Hou T., Lei Z., Shimizu K., Zhang Z.
    • Journal Title

      Science of the Total Environment

      Volume: 783 Pages: 146966

    • DOI

      10.1016/j.scitotenv.2021.146966

    • Peer Reviewed
  • [Journal Article] Insight into Cr(VI) biosorption onto algal-bacterial granular sludge: Cr(VI) bioreduction and its intracellular accumulation in addition to the effects of environmental factors2021

    • Author(s)
      Yang X., Zhao Z., Zhang G., Hirayama S., Nguyen B.V., Lei Z.*, Shimizu K., Zhang Z.
    • Journal Title

      Journal of Hazardous Materials

      Volume: 414 Pages: 125479

    • DOI

      10.1016/j.jhazmat.2021.125479

    • Peer Reviewed
  • [Journal Article] Ionic response of algal-bacterial granular sludge system during biological phosphorus removal from wastewater2021

    • Author(s)
      Wang J., Lei Z.*, Tian C., Liu S., Wang Q., Shimizu K., Zhang Z., Adachi Y., Lee D.-J.
    • Journal Title

      Chemosphere

      Volume: 264 Pages: 128534

    • DOI

      10.1016/j.chemosphere.2020.128534

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Response and recovery of mature algal-bacterial aerobic granular sludge to sudden salinity disturbance in influent wastewater: Granule characteristics and nutrients removal/accumulation2020

    • Author(s)
      Dong X., Zhao Z., Yang X., Lei Z.*, Shimizu K., Zhang Z., Lee D.-J.
    • Journal Title

      Bioresource Technology

      Volume: 321 Pages: 124492

    • DOI

      10.1016/j.biortech.2020.124492

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Behavior of algal-bacterial granular sludge in a novel closed photo-sequencing batch reactor under no external O2 supply2020

    • Author(s)
      Wang J., Lei Z.*, Wei Y., Wang Q., Tian C., Shimizu K., Zhang Z., Adachi Y., Lee D.-J.
    • Journal Title

      Bioresource Technology

      Volume: 318 Pages: 124190

    • DOI

      10.1016/j.biortech.2020.124190

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Enhanced biosorption of Cr(VI) from synthetic wastewater using algal-bacterial aerobic granular sludge: Batch experiments, kinetics and mechanisms2020

    • Author(s)
      Yang X., Zhao Z., Yu Y., Shimizu K., Zhang Z., Lei Z.*, Lee D.-J.
    • Journal Title

      Separation and Purification Technology

      Volume: 251 Pages: 117323

    • DOI

      10.1016/j.seppur.2020.117323

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Coupling biogas recirculation with FeCl3 addition in anaerobic digestion system for simultaneous biogas upgrading, phosphorus conservation and sludge conditioning2020

    • Author(s)
      Yuan T., Wang Y., Nuramkhaan M., Wang X., Zhang Z., Lei Z.*, Shimizu K., Utsumi M., Adachi Y., Lee D.-J.
    • Journal Title

      Bioresource Technolgy

      Volume: 315 Pages: 123811

    • DOI

      10.1016/j.biortech.2020.123811

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Anaerobic co-digestion of hydrolysate from anaerobically digested sludge with raw waste activated sludge: Feasibility assessment of a new sewage sludge management strategy in the context of a local wastewater treatment plant2020

    • Author(s)
      Zhao J., Hou T., Zhang Z., Shimizu K., Lei Z.*, Lee D.-J.
    • Journal Title

      Bioresource Technology

      Volume: 314 Pages: 123748

    • DOI

      10.1016/j.biortech.2020.123748

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Fast cultivation and harvesting of oil-producing microalgae Ankistrodesmus sp. fed with anaerobic digestion liquor via biogranulation in addition to nutrients removal2020

    • Author(s)
      Wang Q., Shen Q., Wang J., Zhang Y., Zhang Z., Lei Z.*, Shimizu K., Lee D.-J.
    • Journal Title

      Science of The Total Environment

      Volume: 741 Pages: 140183

    • DOI

      10.1016/j.scitotenv.2020.140183

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Supplementation of O2-containing gas nanobubble water to enhance methane production from anaerobic digestion of cellulose2020

    • Author(s)
      Wang X., Yuan T., Lei Z.*, Kobayashi M., Adachi Y., Shimizu K., Lee D.-J., Zhang Z.
    • Journal Title

      Chemical Engineering Journal

      Volume: 398 Pages: 125652

    • DOI

      10.1016/j.cej.2020.125652

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Desorption of hexavalent chromium from active aerobic granular sludge: Effects of operation parameters on granular bioactivity and stability2020

    • Author(s)
      Tian C., Wang D., Wang J., Lei Z.*, Zhang Z., Shimizu K.
    • Journal Title

      Bioresource Technology Reports

      Volume: 11 Pages: 100457

    • DOI

      10.1016/j.biteb.2020.100457

    • Peer Reviewed
  • [Journal Article] Hydrothermal treatment of sewage sludge to produce solid biofuel: Focus on fuel characteristics2020

    • Author(s)
      Silva R.D.V.K., Lei Z.*, Shimizu K., Zhang Z.
    • Journal Title

      Bioresource Technology Reports

      Volume: 11 Pages: 100453

    • DOI

      10.1016/j.biteb.2020.100453

    • Peer Reviewed
  • [Journal Article] Improved methane production from corn straw using anaerobically digested sludge pre-augmented by nanobubble water2020

    • Author(s)
      Wang X., Lei Z.*, Shimizu K., Zhang Z., Lee D.-J.
    • Journal Title

      Bioresource Technology

      Volume: 311 Pages: 123479

    • DOI

      10.1016/j.biortech.2020.123479

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of biogas recirculation strategy on biogas upgrading and process stability of anaerobic digestion of sewage sludge under slightly alkaline condition2020

    • Author(s)
      Zhao J., Hou T., Lei Z., Shimizu K., Zhang Z.
    • Journal Title

      Bioresource Technology

      Volume: 308 Pages: 123293

    • DOI

      10.1016/j.biortech.2020.123293

    • Peer Reviewed
  • [Presentation] Application of Biogas Recirculation in Anaerobic Digestion of Sewage Sludge: Perspectives and Challenges2021

    • Author(s)
      Zhongfang Lei* (invited speaker), Kazuya Shimizu, Zhenya Zhang, Yasuhisa Adachi, Duu-Jong Lee
    • Organizer
      International Conference on Biotechnology for Sustainable Agriculture, Environment and Health
    • Int'l Joint Research / Invited
  • [Presentation] Hydrothermal Treatment of Sewage Sludge to Produce Bio Solid Fuel: Investigation of Combustion Characteristics2021

    • Author(s)
      Rammuni Dilini Vasundara Kumari Silva, Zhongfang Lei*, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Feasibility of Anaerobic Digestion System Coupling Biogas Recirculation with Chemical Addition for Simultaneous Biogas Upgrading, Phosphorus Recovery and Sludge Conditioning2021

    • Author(s)
      Tian Yuan, Yinxin Wang, Marjangul Nuramkhaan, Xuezhi Wang, Zhenya Zhang, Zhongfang Lei*, Kazuya Shimizu, Motoo Utsumi, Yasuhisa Adachi, Duu-Jong Lee
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Simultaneous Oil Production Microalgae Harvesting and Nutrients Removal from Digestion Liquid by Biogranulation Process Using Sequencing Batch Reactor2021

    • Author(s)
      Qian Wang, Qingyue Shen, Jixiang Wang, Yihao Zhang, Zhenya Zhang, Zhongfang Lei*, Kazuya Shimizu
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Effect of Biogas Recirculation on Sewage Sludge Treatment and Methane Production under Slightly Alkaline Condition2021

    • Author(s)
      Jiamin Zhao, Tingting Hou, Zhongfang Lei, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Insight into Cr(VI) Biosorption onto Algal-bacterial Granular Sludge: Cr(VI) Bioreduction and Its Intracellular Accumulation in Addition to the Effects of Environmental Factors2021

    • Author(s)
      Xiaojing Yang, Ziwen Zhao, Guanghao Zhang, Shota Hirayama, Bach Van Nguyen, Zhongfang Lei*, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Supplementation of Nanobubble Water to Enhance Methane Production from Anaerobic Digestion of Corn Straw2021

    • Author(s)
      Xuezhi Wang, Zhongfang Lei*, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Simultaneous Phosphorus Recovery and Methane Production Improvement from Waste Activated Sludge via Anaerobic Digestion2021

    • Author(s)
      Boaiqi Zhang, Zhongfang Lei, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Response and Recovery of Algal-bacterial Aerobic Granular Sludge to Saline Wastewater: Focus on Granular Characteristics and Nutrients Removal/Accumulation2021

    • Author(s)
      Xiaochuan Dong, Ziwen Zhao, Zhongfang Lei*, Zhenya Zhang, Kazuya Shimizu
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Potentials of CO2 Capture and Utilization by Algal-bacterial Granular Sludge-based Wastewater Treatment System2021

    • Author(s)
      Jiaqi Liu, Jixiang Wang, Zhongfang Lei*, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Cr(VI) Biosorption by Algal-bacterial Aerobic Granular Sludge: Contribution of Extracellular Polymeric Substance Fractions and Cr Immobilization in Granules2021

    • Author(s)
      Shota Hirayama, Xiaojing Yang, Guanghao Zhang, Bach Van Nguyen, Zhongfang Lei*, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Application of N2-Nanobubble Water to Anaerobic Co-digestion of Alkaline Lignin and Waste Activated Sludge: Simultaneous Enhancement on Lignin Degradation and Methane Production2021

    • Author(s)
      Xiaojing Yang, Jingmin Nie, Yu Wei, Ziwen Zhao, Kazuya Shimizu, Zhongfang Lei*, Zhenya Zhang
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Effects of Aeration on Start-up and Performance of Microalgae Biogranulation System2021

    • Author(s)
      Yuyi Yang, Qian Wang, Zhongfang Lei*, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Effect of Salinity on Cr (VI) Bioremediation by Algal-bacterial Aerobic Granular Sludge Treating Synthetic Wastewater2021

    • Author(s)
      Bach Van Nguyen, Xiaojing Yang, Guanghao Zhang, Shota Hirayama, Zhongfang Lei*, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Comparison between Bacterial and Algal-bacterial Aerobic granular Sludge during Cr(VI) Biosorption: Response to Bacterial Inhibitor and Electron Donor2021

    • Author(s)
      Guanghao Zhang, Xiaojing Yang, Shota Hirayama, Bach Van Nguyen, Zhongfang Lei*, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Effect of Organic Loading Rate on Dynamic Hexavalent Chromium Adsorption/Desorption Using Aerobic Granular Sludge in Sequencing Batch Reactor2021

    • Author(s)
      Caixing Tian, Di Wang, Jixiang Wang, Zhongfang Lei*, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Interactions between Algae and Bacteria on Phosphorus Removal in Response to Major Ions in Wastewater by Using Algal-bacterial Aerobic Granular Sludge System2021

    • Author(s)
      Jixiang Wang, Zhongfang Lei*, Caixing Tian, Sen Liu, Kazuya Shimizu, Zhenya Zhang, Duu-Jong Lee
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Presentation] Accelerated Formation and Enhanced Biomass Growth of Algal-bacterial Aerobic Granular Sludge through Illumination during Aerobic Phase2021

    • Author(s)
      Yanjun Wei, Jixiang Wang, Zhongfang Lei*, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment
    • Int'l Joint Research
  • [Remarks] Researchmap

    • URL

      https://researchmap.jp/Lei_Zhongfang

  • [Remarks] 筑波大学研究者総覧

    • URL

      https://trios.tsukuba.ac.jp/researcher/0000003223

  • [Remarks] Researchgate

    • URL

      https://www.researchgate.net/profile/Zhongfang_Lei

  • [Remarks] Google scholar

    • URL

      https://scholar.google.com/citations?hl=zh-CN&user=VLVoKUsAAAAJ

URL: 

Published: 2021-12-27  

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