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2021 Fiscal Year Annual Research Report

A Sustainable and Recycling-oriented Wastewater Industry by Maximizing Valuable Resources and Energy Recovery Using the Novel Algal-bacterial Aerobic Granular Sludge Technology

Research Project

Project/Area Number 18H03403
Research InstitutionUniversity of Tsukuba

Principal Investigator

雷 中方  筑波大学, 生命環境系, 准教授 (30634505)

Co-Investigator(Kenkyū-buntansha) 張 振亜  筑波大学, 生命環境系, 教授 (20272156)
清水 和哉  東洋大学, 生命科学部, 教授 (10581613)
水野谷 剛  筑波大学, 生命環境系, 准教授 (10500770)
Project Period (FY) 2018-04-01 – 2022-03-31
KeywordsAlgal-bacterial granule / Enhanced P removal / Extracellular polymers / Heavy metal recovery
Outline of Annual Research Achievements

During Year 2021, we finished the following works.
(1)Realized simultaneous recovery of phosphorus (P) and alginate-like exopolymers (ALE) from algal-bacterial aerobic granular sludge (AGS);
(2) Proposed and tested a new efficient P recovery strategy by extracting the P-rich liquid during the anaerobic period when operating the algal-bacterial AGS system;
(3)Made clear the role of extracellular polymeric substances (EPS) during the biogranulation of single cell microalgae; and
(4)Quantified the heavy metals recovery potential of algal-bacterial AGS and verified the safety of the posttreatment of heavy metal-loaded algal-bacterial AGS by using hydrothermal treatment.
We published 15 peer-reviewed high quality journal papers and 18 conference presentations on the above 4 aspects.

Research Progress Status

令和3年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和3年度が最終年度であるため、記入しない。

  • Research Products

    (37 results)

All 2022 2021 Other

All Journal Article (15 results) (of which Int'l Joint Research: 12 results,  Peer Reviewed: 15 results) Presentation (18 results) (of which Int'l Joint Research: 18 results,  Invited: 4 results) Remarks (4 results)

  • [Journal Article] Changes of distribution and chemical speciation of metals in hexavalent chromium loaded algal-bacterial aerobic granular sludge before and after hydrothermal treatment2022

    • Author(s)
      Yang X., Nyuyen B.V., Zhao Z., Wu Y., Lei Z.*, Zhang Z., Le X.S., Lu H.
    • Journal Title

      Bioresource Technology

      Volume: 355 Pages: 127229

    • DOI

      10.1016/j.biortech.2022.127229

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Microalgal-bacterial granular sludge for municipal wastewater treatment: From concept to practice2022

    • Author(s)
      Zhang X., Lei Z., Liu Y.
    • Journal Title

      Bioresource Technology

      Volume: 354 Pages: 127201

    • DOI

      10.1016/j.biortech.2022.127201

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Insight into aerobic phosphorus removal from wastewater in algal-bacterial aerobic granular sludge system2022

    • Author(s)
      Li Z., Wang J., Chen X., Lei Z.*, Yuan T., Shimizu K., Zhang Z., Lee D.-J.
    • Journal Title

      Bioresource Technology

      Volume: 352 Pages: 127104

    • DOI

      10.1016/j.biortech.2022.127104

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Biogranulation process facilitates cost-efficient resources recovery from microalgae-based wastewater treatment systems and the creation of a circular bioeconomy2022

    • Author(s)
      Wang Q., Li H., Shen Q., Wang J., Chen X., Zhang Z., Lei Z.*, Yuan T., Shimizu K., Liu Y., Lee D.-J.
    • Journal Title

      Science of the Total Environment

      Volume: 828 Pages: 154471

    • DOI

      10.1016/j.scitotenv.2022.154471

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of stepwise or one-time illumination strategy on the development of algal-bacterial aerobic granular sludge in sequencing batch reactor2022

    • Author(s)
      Wang J., Wei Y., Li Z., Chen X., Lei Z.*, Yuan T., Shimizu K., Zhang Z., Kim S.-H., Lee D.-J.
    • Journal Title

      Bioresource Technology Reports

      Volume: 17 Pages: 100931

    • DOI

      10.1016/j.biteb.2021.100931

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Achieving stably enhanced biological phosphorus removal from aerobic granular sludge system via phosphorus rich liquid extraction during anaerobic period2022

    • Author(s)
      Wang J., Li Z., Wang Q., Lei Z.*, Yuan T., Shimizu K., Zhang Z., Adachi Y., Lee D.-J., Chen R.
    • Journal Title

      Bioresource Technology

      Volume: 346 Pages: 126439

    • DOI

      10.1016/j.biortech.2021.126439

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Simultaneous recovery of phosphorus and alginate-like exopolysaccharides from two types of aerobic granular sludge2022

    • Author(s)
      Chen X., Wang J., Wang Q., Yuan T., Lei Z.*, Zhang Z., Shimizu K., Lee D.-J.
    • Journal Title

      Bioresource Technology

      Volume: 346 Pages: 126411

    • DOI

      10.1016/j.biortech.2021.126411

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A review on biogas upgrading in anaerobic digestion systems treating organic solids and wastewaters via biogas recirculation2022

    • Author(s)
      Yuan T., Zhang Z., Lei Z.*, Shimizu K., Lee D.-J.
    • Journal Title

      Bioresource Technology

      Volume: 344 Pages: 126412

    • DOI

      10.1016/j.biortech.2021.126412

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Insight into the rapid biogranulation for suspended single-cell microalgae harvesting in wastewater treatment systems: Focus on the role of extracellular polymeric substances2022

    • Author(s)
      Wang Q., Shen Q., Wang J., Zhao J., Zhang Z., Lei Z.*, Yuan T., Shimizu K., Liu Y., Lee D.-J.
    • Journal Title

      Chemical Engineering Journal

      Volume: 430 Pages: 132631

    • DOI

      10.1016/j.cej.2021.132631

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of salinity on Cr(VI) bioremediation by algal-bacterial aerobic granular sludge treating synthetic wastewater2021

    • Author(s)
      Nguyen B.V., Yang X., Hirayama S., Wang J., Zhao Z., Lei Z.*, Shimizu K., Zhang Z., Le S.X.
    • Journal Title

      Processes

      Volume: 9 Pages: 1400

    • DOI

      10.3390/pr9081400

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Application of biogas recirculation in anaerobic granular sludge system for multifunctional sewage sludge management with high efficacy energy recovery2021

    • Author(s)
      Zhao J., Hou T., Wang Q., Zhang Z., Lei Z.*, Shimizu K., Guo W., Ngo H.H.
    • Journal Title

      Applied Energy

      Volume: 298 Pages: 117212

    • DOI

      10.1016/j.apenergy.2021.117212

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cr(VI) bioremediation by active algal-bacterial aerobic granular sludge: Importance of microbial viability, contribution of microalgae and fractionation of loaded Cr2021

    • Author(s)
      Yang X., Zhao Z., Nguyen B.V., Hirayama S., Tian C., Lei Z.*, Shimizu K., Zhang Z.
    • Journal Title

      Journal of Hazardous Materials

      Volume: 418 Pages: 126342

    • DOI

      10.1016/j.jhazmat.2021.126342

    • Peer Reviewed
  • [Journal Article] Recent advancements in nanobubble water technology and its application in energy recovery from organic solid wastes towards a greater environmental friendliness of anaerobic digestion system2021

    • Author(s)
      Wang X., Lei Z.*, Shimizu K., Zhang Z., Lee D.-J.
    • Journal Title

      Renewable and Sustainable Energy Reviews

      Volume: 145 Pages: 111074

    • DOI

      10.1016/j.rser.2021.111074

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Performance and stability of biogas recirculation-driven anaerobic digestion system coupling with alkali addition strategy for sewage sludge treatment2021

    • Author(s)
      Zhao J., Hou T., Lei Z., Shimizu K., Zhang Z.
    • Journal Title

      Science of the Total Environment

      Volume: 783 Pages: 146966

    • DOI

      10.1016/j.scitotenv.2021.146966

    • Peer Reviewed
  • [Journal Article] Insight into Cr(VI) biosorption onto algal-bacterial granular sludge: Cr(VI) bioreduction and its intracellular accumulation in addition to the effects of environmental factors2021

    • Author(s)
      Yang X., Zhao Z., Zhang G., Hirayama S., Nguyen B.V., Lei Z.*, Shimizu K., Zhang Z.
    • Journal Title

      Journal of Hazardous Materials

      Volume: 414 Pages: 125479

    • DOI

      10.1016/j.jhazmat.2021.125479

    • Peer Reviewed
  • [Presentation] Performance of algal-bacterial granular sludge on wastewater treatment under no external O2 supply2021

    • Author(s)
      Zhongfang Lei* (Keynote speaker), Jixiang Wang, Ziwen Zhao, Tian Yuan, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      International Conference on Biotechnology for Resource Efficiency, Energy, Environment, Chemicals and Health (BREEECH 2021)
    • Int'l Joint Research / Invited
  • [Presentation] Granulation of Microalgae and Its Potential for Energy and Resource Recovery from Wastewater2021

    • Author(s)
      Zhongfang Lei* (Keynote speaker)
    • Organizer
      The International Conference on Sustainable Technology and Development (virtual), Elsevier
    • Int'l Joint Research / Invited
  • [Presentation] Rapid cultivation and harvesting of single-cell microalgae via biogranulation: Performance and perspectives2021

    • Author(s)
      Zhongfang Lei*(Keynote speaker), Qian Wang, Tian Yuan, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The 9th International Forum on Industrial Bioprocessing (IBA-IFIBIOP 2021)
    • Int'l Joint Research / Invited
  • [Presentation] Application of Biogas Recirculation in Anaerobic Digestion of Sewage Sludge: Perspectives and Challenges2021

    • Author(s)
      Zhongfang Lei* (Keynote speaker), Kazuya Shimizu, Zhenya Zhang, Yasuhisa Adachi, Duu-Jong Lee
    • Organizer
      International Conference on Biotechnology for Sustainable Agriculture, Environment and Health (BSAEH-2021)
    • Int'l Joint Research / Invited
  • [Presentation] Comparative Study of Microalgae Cultivation and Harvesting between Biogranulation and Suspended Systems: Energy Efficiency and Sustainability Assessment2021

    • Author(s)
      Qian Wang, Hui Li, Qingyue Shen, Jixiang Wang, Zhenya Zhang, Zhongfang Lei*, Tian Yuan, Kazuya Shimizu, Yu Liu
    • Organizer
      The International Workshop on Agricultural Waste Reclamation and Utilization
    • Int'l Joint Research
  • [Presentation] Achieving Stably Enhanced Biological Phosphorus Removal from Aerobic Granular Sludge System via Phosphorus Rich Liquid Separation During Anaerobic Period2021

    • Author(s)
      Jixiang Wang, Zejiao Li, Qian Wang, Zhongfang Lei*, Tian Yuan, Kazuya Shimizu, Zhenya Zhang, Yasuhisa Adachi, Duu-Jong Lee, Rongzhi Chen
    • Organizer
      The International Workshop on Agricultural Waste Reclamation and Utilization
    • Int'l Joint Research
  • [Presentation] Roles of Algae in Phosphorus Removal from Wastewater by Algal-bacterial Aerobic Granular Sludge2021

    • Author(s)
      Zejiao Li, Jixiang Wang, Zhongfang Lei*, Tian Yuan, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The International Workshop on Agricultural Waste Reclamation and Utilization
    • Int'l Joint Research
  • [Presentation] Comparative Study on Simultaneous Recovery of Phosphorus and Alginate-like Exopolysaccharides from Bacterial Aerobic Granular Sludge and Algal-bacterial Aerobic Granular Sludge2021

    • Author(s)
      Xingyu Chen, Jixiang Wang, Qian Wang, Zhongfang Lei*, Zhenya Zhang, Kazuya Shimizu, Tian Yuan
    • Organizer
      The International Workshop on Agricultural Waste Reclamation and Utilization
    • Int'l Joint Research
  • [Presentation] Effect of Influent P Concentration on Formation, Stability and Nutrients Removal of Microalgae Granules in Sequencing Batch Reactor2021

    • Author(s)
      Hui Li, Qian Wang, Xingyu Chen, Zhongfang Lei*, Tian Yuan, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The International Workshop on Agricultural Waste Reclamation and Utilization
    • Int'l Joint Research
  • [Presentation] Removal of Trimethoprim from Pharmaceutical Industrial Wastewater by Algal-bacterial Aerobic Granular Sludge2021

    • Author(s)
      Xingxia Zhou, Zhenya Zhang, Zhongfang Lei*, Kazuya Shimizu, Tian Yuan
    • Organizer
      The International Workshop on Agricultural Waste Reclamation and Utilization
    • Int'l Joint Research
  • [Presentation] Bioremediation of Wastewater Containing Pb(II) by Living Algal-Bacterial Aerobic Granule Sludge: Performance Optimization and Mechanism Insight2021

    • Author(s)
      Zhiwei Wang, Hanxiao Wang, Qi Nie, Zhongfang Lei*, Zhenya Zhang, Tian Yuan, Kazuya Shimizu
    • Organizer
      The International Workshop on Agricultural Waste Reclamation and Utilization
    • Int'l Joint Research
  • [Presentation] Performance of Algal-bacterial Aerobic Granule Sludge on the Treatment of Surfactant-containing Wastewater2021

    • Author(s)
      Hanxiao Wang, Zhiwei Wang, Yi Ding, Tongtong Liu, Zhenya Zhang, Zhongfang Lei*, Tian Yuan, Kazuya Shimizu
    • Organizer
      The International Workshop on Agricultural Waste Reclamation and Utilization
    • Int'l Joint Research
  • [Presentation] Granulation Initialed by Two Microalgal Strains Isolated from Algal-bacterial Granular Sludge and Their Contribution to Nutrients Removal and Dynamic Changes of Biological Community2021

    • Author(s)
      Marjangul Nuramkhaan, Xiaochuan Dong, Zhongfang Lei*, Kazuya Shimizu, Zhenya Zhang, Motoo Utsumi
    • Organizer
      The International Workshop on Agricultural Waste Reclamation and Utilization
    • Int'l Joint Research
  • [Presentation] Effect of Aeration Intensity on the Start-up and Performance of Microalgae Granulation System under Intermittent Aeration Condition2021

    • Author(s)
      Qian Wang, Yuyi Yang, Qingyue Shen, Jixiang Wang, Zhenya Zhang, Zhongfang Lei*, Tian Yuan, Kazuya Shimizu, Yu Liu
    • Organizer
      The International Workshop on Agricultural Waste Reclamation and Utilization
    • Int'l Joint Research
  • [Presentation] Accelerated Formation and Enhanced Biomass Growth of Algal-bacterial Aerobic Granular Sludge through Illumination during Aerobic Phase2021

    • Author(s)
      Yanjun Wei, Jixiang Wang, Zhongfang Lei*, Kazuya Shimizu, Zhenya Zhang
    • Organizer
      The International Workshop on Agricultural Waste Reclamation and Utilization
    • Int'l Joint Research
  • [Presentation] Response and Recovery of Mature Algal-bacterial Aerobic Granular Sludge to Sudden Salinity Disturbance in Influent Wastewater: Granule Characteristics and Nutrients Removal/Accumulation2021

    • Author(s)
      Xiaochuan Dong, Ziwen Zhao, Xiaojing Yang, Zhongfang Lei*, Kazuya Shimizu, Zhenya Zhang, Duu-Jong Lee
    • Organizer
      The International Workshop on Agricultural Waste Reclamation and Utilization
    • Int'l Joint Research
  • [Presentation] Cr(VI) Bioremediation by Active Algal-bacterial Aerobic Granular Sludge: Importance of Microbial Viability, Contribution of Microalgae and Fractionation of Loaded Cr2021

    • Author(s)
      Xiaojing Yang, Bach Van Nguyen, Ziwen Zhao, Zhongfang Lei*, Zhenya Zhang, Xuan Sinh Le, Hui Lv
    • Organizer
      The International Workshop on Agricultural Waste Reclamation and Utilization
    • Int'l Joint Research
  • [Presentation] Enhanced Stability of Aerobic Granular Sludge System to Salinity Stress by Adding Crushed Algal-bacterial Aerobic Granular Sludge2021

    • Author(s)
      Ziwen Zhao, Xiaojing Yang, Xiaochuan Dong, Wei Cai, Zhongfang Lei*, Zhenya Zhang, Duu-Jong Lee
    • Organizer
      The International Workshop on Agricultural Waste Reclamation and Utilization
    • Int'l Joint Research
  • [Remarks] 筑波大学研究者総覧 TRIOS Researchers Information

    • URL

      https://trios.tsukuba.ac.jp/en/researchers/0000003223

  • [Remarks] Researchmap

    • URL

      https://researchmap.jp/Lei_Zhongfang

  • [Remarks] Google Scholar

    • URL

      https://scholar.google.com/citations?user=VLVoKUsAAAAJ&hl=en

  • [Remarks] Researchgate

    • URL

      https://www.researchgate.net/profile/Zhongfang-Lei

URL: 

Published: 2022-12-28  

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