2021 Fiscal Year Final Research Report

Induction of vasculogenesis and angiogenesis in three-dimensional tissues by using protein expressions with mRNA delivery

Research Project

PDF

Project/Area Number	18H03537
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 90120:Biomaterials-related
Research Institution	Tokyo Women's Medical University
Principal Investigator	Kobayashi Jun 東京女子医科大学, 医学部, 講師 (20385404)
Co-Investigator(Kenkyū-buntansha)	秋山義勝東京女子医科大学, 医学部, 講師 (20349640) 増田信奈子東京女子医科大学, 医学部, 助教 (30342851) 中山正道東京女子医科大学, 医学部, 講師 (00338980) 辰巳公平奈良県立医科大学, 医学部, 准教授 (70555432) 山岡哲二国立研究開発法人国立循環器病研究センター, 研究所, 部長 (50243126) 長瀬健一慶應義塾大学, 薬学部(芝共立), 准教授 (10439838)
Project Period (FY)	2018-04-01 – 2022-03-31
Keywords	細胞シート / 血管新生 / 脈管形成 / mRNA送達
Outline of Final Research Achievements	In this study, we developed a novel technique to induce angiogenesis in 3D cell sheet tissue and promote angiogenesis to connect with a host blood vessel. By introducing VEGF-encoding messenger RNA (mRNA) into the 3D cell sheet tissue and allowing it to act like a paracline in the vicinity of vascular endothelial cells, it was suggested to induce angiogenesis inside the 3D cell sheet tissue. It was also found that transplantable liver / cardiomyocyte sheets that rapidly secrete VEGF can be prepared by mRNA delivery. Furthermore, by stacking these, it can be expected to be applied to the construction of a functional three-dimensional tissue having a capillary-like structure of endothelial cells.
Free Research Field	組織工学
Academic Significance and Societal Importance of the Research Achievements	mRNAを利用した脈管形成と同様の内容はこれまでに報告されていない。また、移植可能な3D組織の構築が実現されれば、これまでに不可能であった肝組織の効率的な移植方法につながり、組織再生治療分野における大きなブレークスルーとなると同時に、肝組織再生治療の臨床応用への展開が期待される。