2021 Fiscal Year Final Research Report
Plant Glycosignaling Based on the Discovery of AMOR, a Bioactive Sugar Chain for Intercellular Signaling
| Project/Area Number |
18H03997
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Keywords | 糖鎖 / 植物 / 細胞間シグナリング / 受容体 / 有機化学合成 |
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| Outline of Final Research Achievements |
The aim of this study was to clarify the importance of AMOR as an signaling molecule in plant cell-to-cell communication and how it is used for signal transduction, using the biologically active sugar chain AMOR. In order to explore the new functions of AMOR and to analyze the mechanism of AMOR reception, we succeeded in modifying AMOR by organic chemical synthesis. Based on this, we established monoclonal antibodies that specifically recognize the AMOR structure (methylglucuronosylgalactose). In addition, multimeric AMOR molecules were produced and high activity per molecule was confirmed; it is expected that Torenia crustacea will be useful for further analysis of the AMOR synthesis system and candidate receptor genes in combination of developed molecular tools.
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| Free Research Field |
分子細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
AMORは特定の生理活性を有する植物糖鎖構造として初めて同定された。有機化学合成により植物糖鎖の研究を進めている研究グループは国内外になく、本研究は目的においてもアプローチにおいても、極めて先導的な取組みとなった。AMOR構造であるメチルグルクロノシルガラクトースを特異的に認識するモノクローナル抗体や、多量体AMORの作製にも初めて成功した。トレニアで見いだされたトレニアのAMOR生合成系や受容体等の候補遺伝子群について、トレニア属ウリクサにおける進展も期待される。植物生殖の研究においても、植物糖鎖シグナリングの研究においても、新たな研究の潮流を生み出す研究として大きな意義がある。
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