2018 Fiscal Year Annual Research Report
Study on rhizosphere of arsenic hyperaccumulators forward to develop an efficient phytoremediation technology
| Project/Area Number |
18J11625
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| Research Institution | Tohoku University |
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Principal Investigator |
YANG CHONGYANG 東北大学, 環境科学研究科, 特別研究員(DC2)
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| Project Period (FY) |
2018-04-25 – 2020-03-31
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| Keywords | arsenic / rhizobacteria / Pteris vittata |
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| Outline of Annual Research Achievements |
The research went smoothly as planned. In this year, I focused on rhizosphere of arsenic hyperaccumulators Pteris vittata and Pteris multifida and role of rhizosphere bacteria to elucidate the interactions of plants and rhizobacteria. The aim of this study was to investigate the potential of rhizobacteria in enhancing the effect of arsenic phytoextraction. Seventy-eight strains rhizobacteria was isolated and identified by blasting to gene bank. Their traits were characterized to select the optimal isolate. The interactions of selected multifunctional strain with plants were exhibited positive effects. Further, co-cultivation of rhizobacterium and ferns achieved the promotion arsenic phytoremediation. I wrote two papers, one was published, another one was under reviewing. According to papers writing, I obtained a deeper understanding of research, which can lay a solid foundation for further research plan. And I attended an international conference and national conference. Academic communication provides the enlighten thoughts and ideas about my research.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The title of my research is “Study on rhizosphere of arsenic hyperaccumulators forward to develop an efficient phytoremediation technology”. I mainly focused on rhizosphere of arsenic hyperaccumulators Pteris vittata and Pteris multifida and role of rhizosphere bacteria to elucidate the interactions of plants and rhizobacteria. The aim of this study was to investigate the potential of rhizobacteria in enhancing the effect of arsenic phytoextraction. And base on it, rhizobacteria was isolated and identified by blasting to gene bank. Their traits were characterized to select the optimal isolate. The interactions of selected multifunctional strain with plants were exhibited positive effects. Further, co-cultivation of rhizobacterium and ferns achieved the promotion arsenic phytoremediation. The research went smoothly as planned. Each experiment was conducted after a lot of relevant literatures investigating, independent thinking and communication with professor or laboratory members.
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| Strategy for Future Research Activity |
(1)Molecular analysis will be applied in rhizosphere samples of co-cultivation field trail. A aioA operon which promotes As(III) to As(V) will be detected as target gene to calculate the abundance of bacteria with aio gene in rhizosphere after the inoculation.
(2) Pot experiment will be applied to control needless variable in laboratory. Next generation sequence will be analyzed to detect the microbial diversity and richness with functional strain inoculation and non-inoculation.
(3)Summarized 3 years of phytoremediation with functional strain inoculation. Soil arsenic will be detected to compare changeable of 3 years phytoremediation. Proposal of long-term phytoremediation will be offered and optimized for further study.
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