2020 Fiscal Year Annual Research Report
Study on metabolism/physiological functions of retinoic acid in metamorphic larvae of the Pleuronectiformes
| Project/Area Number |
18J20627
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| Research Institution | Tohoku University |
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Principal Investigator |
陳 其然 東北大学, 農学研究科, 特別研究員(DC1)
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| Project Period (FY) |
2018-04-25 – 2021-03-31
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| Keywords | RNA-Seq / Retinoic acid / cyp26 / Plasmid delivery / Electroporation / Japanese flounder / bco1 |
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| Outline of Annual Research Achievements |
1. RT-PCR confirmed bco1l was down-regulated by RA treatment in the intestine. Members of cyp26 and dhrs3 were up-regulated in both sides of skin, vertebra and gills. The data suggest malformations caused by RA exposure is systemically related to this mechanism.
2. The success of transferring plasmid to flounder tissues and bamboo sole ovary eggs provides the basis for technique using CRISPR/Cas9 system in flatfish, which distribute to developmental biology in asymmetry study.
3. Genes encoding retinoic acid receptors expressed ubiquitously in G-stage flounders. The expression of rars in both sides of skin and vertebra suggest the skins and vertebra are responsive to RA induction. Bone and skin malformation caused by RA induction at G-stage is related to the expression of these rars.
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| Research Progress Status |
令和2年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和2年度が最終年度であるため、記入しない。
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