2018 Fiscal Year Research-status Report
New quantum biosensors for quantitative molecular imaging assays of oxidative stress
| Project/Area Number |
18K04909
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| Research Institution | National Institutes for Quantum and Radiological Science and Technology |
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Principal Investigator |
Rumiana Bakalova 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 分子イメージング診断治療研究部, 主任研究員(任常) (30469936)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | redox / EPR / MRI / hypercholesterolemia / fluorescent imaging |
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| Outline of Annual Research Achievements |
We synthesized “oxidative stress” sensor (OxiStressSens) based on conjugation of quantum dots with multi-nitroxide-functionalized cyclodextrin. The oxidation of nitroxide residues to their radical form was accompanied by strong quenching of quantum dot fluorescence and appearance of high EPR contrast. In opposite, the reduction of nitroxide residues in their hydroxylamine form was accompanied by rapid decay of EPR signal and appearance of strong fluorescence signal. Thus, OxyStressSens allows evaluation of the balance between reducers/oxidizers and level of “oxidative stress”.
The sensor enters into viable cells, which was visualized by EPR and fluorescent imaging. OxyStressSens was applied for detection of “oxidative stress” in cell suspensions, treated by rotenone and 2-methoxyestradiol; a combination, inducing mitochondrial dysfunction, overproduction of superoxide and mild or severe oxidative damages, as well as on cell with different proliferation index. The physicochemical characteristics and easy cell permeability give a reason to believe that the sensor will be appropriate for in vivo imaging. Since the T1-weigheted MRI contrast of nitroxides follows the same dynamics as their EPR contrast, the sensor is applicable also for nitroxide-enhanced MRI studies.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
I published three papers per year.
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| Strategy for Future Research Activity |
The project is going well. So, I proceed the project according to my initial plan.
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| Causes of Carryover |
少額の端数が生じた。次年度、物品費に算入する。
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