2023 Fiscal Year Final Research Report
Genome mining for silent novel biomass-degrading enzymes from fungi
| Project/Area Number |
18K05383
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Keywords | ゲノムマイニング / Aspergillus oryzae / Glycoside hydrolase / エンドグルカナーゼ / β-グルコシダーゼ / キシラナーゼ / タンナーゼ / バイオマス |
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| Outline of Final Research Achievements |
In this study, from the genes presumed to encode cellulases on the Aspergillus oryzae genome using the genome mining method, unanalyzed genes were selected using various databases, and high expression strains in A. oryzae were generated and analyzed. Among them, we found that the putative endoglucanase EglA was an enzyme that retained unique activity, the putative β-glucosidase (Bgl) BglU/Bgl5 was an enzyme that was highly resistant to activity inhibition by glucose (reaction product), which was the biggest problem of Bgl, and the putative xylanase XynB was an enzyme that retained high properties of both optimum temperature and temperature stability.
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| Free Research Field |
応用微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、ゲノムマイニングの手法によって、推定バイオマス分解酵素遺伝子を麹菌ゲノムから網羅的に探索し、EglA、BglU/Bgl5、XynBといった有用な新規バイオマス分解酵素を取得できた。新規有用酵素の探索は、酵素を生産する微生物の発掘が従来研究の主流であったが、新たにゲノムマイニング法によって新規有用酵素の取得が可能であることが判明し、成果の波及効果は極めて大きいものと考えられる。また、本研究で取得できたBglU/Bgl5は、報告例の少ないグルコース(反応生成物)による活性阻害に対して耐性が高い酵素であったことから、今後社会実装化が期待される。
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