2020 Fiscal Year Final Research Report
Elucidation of hemostasis control mechanism involving extracellular vesicles released by amphbian thrombocytes
| Project/Area Number |
18K05552
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38060:Applied molecular and cellular biology-related
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | 栓球細胞 / 細胞外小胞 / CD41 / マイクロRNA / 血管内皮細胞 / マイクロアレイ |
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| Outline of Final Research Achievements |
Many detailed characteristics of thrombocytes remain unclear. Here, we report the finding that thrombocytes produce integrin alpha IIb (CD41)-positive extracellular vesicles (EVs), which include microRNAs (miRs). FCM analysis revealed the expression of CD41+ on the surface of EVs. Nanotracking analysis demonstrated that these CD41+ EVs were approximately 100 nm in diameter. Microarray analysis revealed that the CD41+ EVs contain many kinds of miRs. These CD41+ EVs were phagocytosed by endothelial cells and macrophages. qPCR analysis revealed that many angiogenesis-related genes were upregulated in CD41+ EV-treated endothelial cells. These results indicated that thrombocytes produced CD41+ EVs, including miRs, that were received by endothelial cells to induce the expression of angiogenesis-related genes. These results indicated that the CD41+ EVs produced from thrombocytes act as signaling molecules to repair damaged blood vessels.
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| Free Research Field |
細胞生物学，分子生物学，血液学
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| Academic Significance and Societal Importance of the Research Achievements |
海外を含めてこれまで両生類栓球細胞株は樹立されていませんでした。今回，栓球細胞株を樹立することにより初めて，栓球細胞がCD41陽性細胞外小胞を産生すること，この中のマイクロRNAを特定すること，またこのマイクロRNAが内皮細胞の増殖制御に関わるといった機能を明らかにすることが出来ました。マイクロRNAのデータを比較すると、哺乳類血小板にも同じようなマイクロRNAが存在するため，これらのマイクロRNAは進化的に保存されてきた生理機能制御に関わる重要なマイクロRNAである事が判ります。これは我々ヒトを含めた哺乳類血小板がもつマイクロRNAを，血管新生制御に利用出来る可能性を示唆するものです。
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