2020 Fiscal Year Final Research Report
Positional cloning and functional analysis of the genes responsible for lethal mutation in the silkworm, Bombyx mori
| Project/Area Number |
18K05672
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 39050:Insect science-related
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Ito Katsuhiko 東京農工大学, (連合)農学研究科(研究院), 准教授 (80725812)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Keywords | カイコ / 突然変異 / 致死 / ポジショナルクローニング / ゲノム編集 |
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| Outline of Final Research Achievements |
The aim of this study is to identify the lethal genes that are expressed at three different growth stages, egg, hatching, and molting stages. In the l-19 gene responsible for egg lethal, we performed the positional cloning and successfully narrowed down the l-19 locus to a region approximately 200 kb long on chromosome 12. Moreover, we found one candidate gene for the l-19 gene. In the l-nl gene responsible for lethality immediately after hatching, we demonstrated that one best candidate gene is responsible for the l-nl phenotype through positional cloning and genome editing approaches. In the nm-d gene responsible for abnormal molting, we delimited the nm-d locus to an approximately 2.7 Mb region on chromosome 9.
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| Free Research Field |
昆虫遺伝学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、カイコを従来の養蚕業上重要な昆虫として位置づけるのではなく、作物の害虫であるチョウ目昆虫として考え、致死という表現型をペストコントロールに展開するという目的をもつ。研究成果の学術的意義は、得られる成果が昆虫の生死に関わる新たな重要な遺伝子の発見につながる点である。また、研究成果の社会的意義は、明らかにした生命現象をペストコントロールに応用することで作物保護につながる点である。
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