Development of production method of high-optically pure lactic acid from non-sterilized biomass derived from rice manufacturing process by the arbitrary control of microbial flora in SSF.

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K05920
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 41050:Environmental agriculture-related
• Research Institution: Yamagata University
• Principal Investigator: Watanabe Masanori 山形大学, 農学部, 教授 (20320020)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 光学活性乳酸 / 米加工副産物 / T-RFLP法 / バイオマスリファイナリー

Outline of Final Research Achievements

In this study, we evaluate the high-optically pure lactic acid production from non-sterilized biomass derived from rice by a newly isolated high-optically pure lactic acid producing bacterium (L. rhamnosus M23) under SSF cultivation. Antibacterial peptides were not produced in SSF culture. From the results of the T-RFLP analysis of SSF microbial flora, High lactic acid production was attained by the synergetic effect of decrease of dominance formation of Clostridium sp. cluster IX, XIVa and Bacteroidales, low accumulation of lower fatty acids and ethanol by the addition of acidic protease, pH control and oxygen partial supply SSF.

Free Research Field

バイオマス資源学

Academic Significance and Societal Importance of the Research Achievements

本課題は，SSF発酵液中のバイオマス表層構造，菌体外プロテアーゼ等酵素系の挙動及び機能，微生物群集構造などの複合的要因と乳酸生成能の関係を解明し，「米副産物を原料とした環境調和型高光学活性乳酸製造技術を確立する」点に学術的特徴がある。本研究の実施により，米由来バイオマスを非加熱・無殺菌状態のまま，乳酸製造の炭素源，栄養源，糖化酵素供給源として安定的に利用可能となれば，当該バイオマスの再資源化の促進のみならず，関連する廃棄物・廃水の減量化，資源・エネルギー循環社会形成等，産業化にも社会的にもその波及効果は極めて大きい。