2020 Fiscal Year Final Research Report
Identification of novel and powerful constitutive promoter than CAG
Project/Area Number |
18K06039
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 42040:Laboratory animal science-related
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Research Institution | Keio University |
Principal Investigator |
NAKATAKE Yuhki 慶應義塾大学, 医学部(信濃町), 講師 (20415251)
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Keywords | プロモーター / 遺伝子発現 / 網羅的解析 / 恒常発現 |
Outline of Final Research Achievements |
To analyze molecular mechanisms, transgene induction is one of the most popular approach in biology field. Well-characterized promoters can induce the downstream gene of interest. Such promoters were established by previous studies, but systematic approaches have not been applied to identify the best candidates for it. In this study, transcriptome data was utilized for the identification of the candidate that could induce downstream genes. The identified gene promoters in this study retained a significant transcriptional activity, but not superior active than CAG promoter that is classically used in this field. These observations suggest that structure of promoter is more important than sequence themselves.
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Free Research Field |
分子生物学
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Academic Significance and Societal Importance of the Research Achievements |
古典的に使用されている分子生物学的ツールは、特段に不都合が無ければ、あまり着目されることもなく、盲目的に使用される傾向にある。ただ、実験目的によっては、既存のツールがうまく機能せず、得たい実験結果が得られない等のトラブルが生じる。本研究は、そのような申請者の実経験から着想され、最新の知見を駆使することで、古い固定概念を覆そうとした。今後、本知見を踏まえ、さらに良いツールの開発がされれば、今までできなかった実験評価が実施できるなど、生物学の分子メカニズムの解明への貢献が期待される。
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