Analysis of centriole duplication process

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K06233
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44010:Cell biology-related
• Research Institution: The University of Tokyo
• Principal Investigator: Takao Daisuke 東京大学, 大学院医学系研究科(医学部), 助教 (10548811)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 中心体 / 分子ダイナミクス / 超解像イメージング / 数理モデル

Outline of Final Research Achievements

Centrioles are duplicated to produce a single copy of each preexisting centriole. At the onset of centriole duplication, the master regulator Plk4 undergoes a dynamic change in its spatial pattern around the preexisting centriole, forming a single duplication site. However, the significance and mechanisms of this pattern transition remain unknown. Based on results from super-resolution imaging and mathematical modeling, we proposed that the self-patterning of Plk4 is crucial for the regulation of centriole duplication. These results, defining the mechanisms of self-organized regulation, provide a fundamental principle for understanding centriole duplication.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

中心体の数を厳密に制御するメカニズムは正常な細胞分裂に重要であり、そのメカニズムの異常は癌化に関連することも指摘されている。このような重要な細胞機能の一端を解明できたことは、今後の生物学・医学における重要な進展である。