Elucidation of mechanisms for development, maturation, and maintenance in hematopoietic stem cells of intra-aortic hematopoietic cell clusters at midgestated mouse embryos

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K06249
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44020:Developmental biology-related
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: NOBUHISA IKUO 東京医科歯科大学, 難治疾患研究所, 准教授 (40332879)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 造血幹細胞 / 転写因子 / 血液細胞塊 / Sox17 / 胎仔

Outline of Final Research Achievements

Hematopoietic stem cells, which are the source of all blood cells, first arise in hematopoietic cell clusters of the dorsal aorta in the embryonic day 10.5 of the mouse embryo. We analyzed genes and signaling pathways induced by Sox17, a transcription factor involved in the development, maturation and maintenance of hematopoietic stem cells in midgestation mouse embryos. We investigated the involvement of adherent molecules ESAM, the GTPase Gimap6, the signaling pathway activating NF-kappaB, and TET1 involved in DNA methylation, respectively.

Free Research Field

発生生物学

Academic Significance and Societal Importance of the Research Achievements

今回得られたマウス胎生中期における大動脈内腔の造血幹細胞を含む血液細胞塊の形成・成熟・維持にかかわる分子およびシグナル経路をさらに解析していくことにより、生体の造血幹細胞の特性と比較することにより、培養皿上での造血幹細胞の形成および維持機構の解明につながることが期待される。