Study for wound epidermis signal to regulate regeneration

2021 Fiscal Year Final Research Report

• Project/Area Number: 18K06266
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44020:Developmental biology-related
• Research Institution: University of Hyogo
• Principal Investigator: Mochii Makoto 兵庫県立大学, 理学研究科, 准教授 (90202358)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Keywords: アフリカツメガエル / 再生 / 傷表皮 / apical epithelial cap / 分泌性シグナル因子

Outline of Final Research Achievements

Wound epidermis (WE) and the apical epithelial cap (AEC) are believed to trigger regeneration of amputated appendages such as limb and tail in amphibians by producing certain secreted signaling molecules. Here we used an es1:egfp transgenic Xenopus laevis to isolate WE/AEC cells during the time course of tail regeneration. Transcriptome analysis revealed that more than 8,000 genes, including genes involved in several signaling pathways, displayed dynamic changes of their expression during tail regeneration. We identify seven secreted signaling molecule genes (mdk, fstl, slit1, tgfβ1, bmp7.1, angptl2 and egfl6) that are highly expressed in tail AEC cells. Among these genes, five were also highly expressed in limb AEC cells but the other two are specifically expressed in tail AEC cells. Interestingly, there was no expression of fgf8 in tail WE/AEC cells, whose expression and pivotal role in limb AEC cells have been reported previously.

Free Research Field

発生生物学

Academic Significance and Societal Importance of the Research Achievements

本研究では、尾部の傷表皮/AECが比較的多種類の分泌因子を放出することおよび、尾部と肢芽とで分泌因子の組成が異なることを明らかにした。この発見は器官再生のメカニズム解明へ向けての重要なステップとなる 。