2021 Fiscal Year Final Research Report
Role of CD34+ endothelial cells in in vivo testis formation and in vitro reconstruction of testis
| Project/Area Number |
18K06322
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44040:Morphology and anatomical structure-related
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| Research Institution | Kumamoto Health Science University |
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Principal Investigator |
Abe Shin-ichi 熊本保健科学大学, 保健科学部, 客員研究員 (90109637)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Keywords | 精巣形成 / 3次元培養 / organoid / 間質 / CD34 / telocyte / integrin a4b1 / VCAM1 |
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| Outline of Final Research Achievements |
We analyzed the mechanism whereby Leydig cells (LCs) are localized within the re-aggregates of CD34+ cells, which are the main component of the testicular interstitium, in a 3-D re-aggregate culture in which successful reconstruction of testicular structures had been established. While LCs have adult type (ALCs) and fetal type (FLCs), most of the LCs localized within the CD34+ cell-re-aggregates were ALCs. Live cell imaging showed that CD34+ cells on the dish displayed chemotactic behavior toward ALC-re-aggregates, and the behavior was inhibited by VCAM1 antibody. Combined with expression studies by immunostaining, these results indicate that VCAM1-a4b1 integrin interaction between CD34+ cells (telocytes) and ALCs plays an important role in the formation, maintenance and regeneration of the structure of testicular interstitium. The testicular organoid model that we established will contribute to the analysis of the mechanism of spermatogenesis and also to medical application.
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| Free Research Field |
発生生物学
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| Academic Significance and Societal Importance of the Research Achievements |
近年、精巣を含め哺乳類のいろいろな器官の間質にCD34+PDGFRa+ telocytesが存在し、周りの細胞とcommunicationを取っているという形態学的報告がある。再凝集培養系を用いて、精巣の間質様構造の再構築にCD34+細胞とadult LC間のVCAM1-a4b1 integrin相互作用が重要な役割を果たしていることを示唆した今回の結果は、生体におけるLCの分化・再生におけるtelocytesの機能的役割を示唆する先駆的な報告と言えるだろう。また、我々が確立した精巣organoidが精巣再構築の解析に有用なツールとなることが証明されたことから、今後医学的な応用も期待される。
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