Axonal regeneration through Photoactivated adenylyl cyclase

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K06482
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 46010:Neuroscience-general-related
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Omura Takao 浜松医科大学, 医学部附属病院, 講師 (70402295)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 神経再生 / 中枢神経損傷 / PAC

Outline of Final Research Achievements

We overexpressed Photoactivated adenylyl cyclase(PAC) in the DRG sensory neurons　through AAV and analyzed axonal growth and neuronal sprouting in comparison with the control AAV. WE found that activation of PAC significantly increased axonal growth and sprouting in the DRG neurons. We next used in vivo optic nerve injury model to see whether PAC was able to promote axonal growth in the CNS. WE discovered that activation of PAC though AAV induced axonal growth after optic nerve injury, when no growth was observed in the control virus group/

Free Research Field

整形外科

Academic Significance and Societal Importance of the Research Achievements

脳梗塞、脊髄損傷などの中枢神経損傷に対する有効な治療法は確立されておらず、これら疾患から回復することは極めて困難である。我々の研究ではPACを介し、中枢神経の損傷モデルである視神経損傷において軸索再生の促進が見られたことにより、今後中枢神経損傷患者に対する治療の一助となる可能性が示唆された。