Multi-omics analysis of brain exosomes on drug abuse

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K06674
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47030:Pharmaceutical hygiene and biochemistry-related
• Research Institution: Kobe Gakuin University
• Principal Investigator: Yamashita Takuya 神戸学院大学, 薬学部, 助教 (10645203)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: Exosome / 危険ドラッグ / 統合オミクス

Outline of Final Research Achievements

Anti-inflammatory related proteins (Thioredoxin-like protein 1, Latexin) expression were incleased in exosomes derived human nerve cells on 4-methoxy amphetamine (4-MeO AP) abuse group compare to AP group. Moreover, phosphatidylcholines containing docosahexaenoic acids were reduced in exosomes derived human nerve cells on 4-MeO AP abuse group compare to AP group. In this study, we showed the difference inflammatory response induced by nerve cell-derived exosomes depends on the types of drugs.

Free Research Field

法中毒

Academic Significance and Societal Importance of the Research Achievements

本研究では覚せい剤に類似した構造を持つ薬物が、Exosomeを介して脳内炎症応答を惹起するメカニズムの一端を明らかにした。世界のExosome研究において類似の結果報告はないことから、独自性の高い研究であるといえる。また、危険ドラッグの蔓延は社会問題であり、より強力な法整備を行う上での重要なエビデンスとなるものであり、社会的意義は非常に高い。