Development for neuronal stem cells control compounds using molecular networking

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K06727
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47050:Environmental and natural pharmaceutical resources-related
• Research Institution: Tokushima Bunri University
• Principal Investigator: Kubo Miwa 徳島文理大学, 薬学部, 准教授 (00330754)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 神経栄養因子様活性物質

Outline of Final Research Achievements

As a primary screening for the discovery of neurotrophic factor-like active substances, the BS test (BST) was applied to screen a library of about 600 plants from Myanmar. As a result, I found that several plant species including Gmelina arborea belonging to the Perillaceae family showed BST activity and they exhibited neurotrophic factor-like activity against PC12 cells. I found that BST, which can be easily performed by anyone, is useful as a primary screening tool to search for neurotrophic factor-like active substances.

Free Research Field

天然物化学

Academic Significance and Societal Importance of the Research Achievements

創薬の初期を担う1次スクリーニングは，安価で，簡便且つ迅速であることが望ましい。本研究では，誰もができるブラインシュリンプアッセイを用いて，世界が渇望するアルツハイマー型認知症の根本的治療予防薬開発を指向した，神経可塑性賦活化剤である神経栄養因子様活性物質探索のための簡便なフェノタイプ型ハイスループットスクリーニングを構築し，天然物メタボロミクスを活用した高効率的な化合物精製法と統合した次世代型天然物創薬の発展へ繋がる結果を得ることができた。