2020 Fiscal Year Final Research Report

Establishment of cytological criteria using image engineering analysis method for early diagnosis of epithelial neoplastic lesions

Research Project

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Project/Area Number	18K07000
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 49020:Human pathology-related
Research Institution	Nihon University
Principal Investigator	KUYAMA Kayo 日本大学, 松戸歯学部, 教授 (00234526)
Co-Investigator(Kenkyū-buntansha)	末光正昌日本大学, 松戸歯学部, 助教 (10708770) 齋藤美雪 (森川美雪) 日本大学, 松戸歯学部, 助教 (90246926) 山本泰日本大学, 松戸歯学部, 講師 (80459586) 田口千恵子日本大学, 松戸歯学部, 助教 (80434091)
Project Period (FY)	2018-04-01 – 2021-03-31
Keywords	口腔細胞診 / 細胞診ガイドライン / 深層型扁平上皮細胞 / 角化型表層異型扁平上皮細胞 / 細胞形態学的画像解析 / 細胞判定基準 / 細胞質の輝度 / 核形態
Outline of Final Research Achievements	The quality control of oral cytology is not developed. The purpose of this study was to clarify the oral cytological criteria based on cytomorphological image analysis as an objective basis.　 The cells to be analyzed were keratinized superficial squamous atypical cells and deep squamous epithelial cells, and cytomorphological and statistical analyzed were performed. In analysis result, increased “cytoplasmic brightness (with significance)” was observed in the keratinized superficial squamous atypical cells in squamous cell carcinoma although there was no difference in nuclear morphology compared to epithelial dysplasia. Concerning about deep squamous epithelial cells, “enhancement densely stained nucleus and nuclear irregularity (with significance)” was observed in epithelial neoplastic lesion compared to inflammation. These findings were reported, and a schematic diagram was created and published.
Free Research Field	口腔病理学
Academic Significance and Societal Importance of the Research Achievements	口腔細胞診は、明文化された細胞判定基準がないまま急速に普及しているために精度管理が行われていない。因って明確な判定基準作りは、偽陰性判定により口腔がん患者が見逃される不利益を防ぐためにも意義が大きい．本研究は、細胞画像解析を客観的根拠とする口腔細胞判定基準の明文化を目的とした。研究の結果、口腔細胞診標本で主体となる角化型表層異型扁平上皮細胞の扁平上皮癌ででの「細胞質の輝度の亢進」、深層型扁平上皮細胞の腫瘍性疾患（扁平上皮癌および上皮性異形成）での「核の濃染性と核形不整の亢進」を発見し、公開した。本研究結果は、口腔がん検診の精度向上につながる社会的意義が非常に多いと考える。