Development of liver regenerative therapy using stem cells from human exfoliated deciduous teeth for clinical application

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K08598
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55010:General surgery and pediatric surgery-related
• Research Institution: Kyushu University
• Principal Investigator: HAYASHIDA Makoto 九州大学, 医学研究院, 共同研究員 (70452761)
• Co-Investigator(Kenkyū-buntansha): 松浦 俊治 九州大学, 大学病院, 准教授 (10532856) ; 田口 智章 九州大学, 医学研究院, 教授 (20197247) ; 吉丸 耕一朗 九州大学, 医学研究院, 講師 (60711190) ; 山座 孝義 九州大学, 歯学研究院, 准教授 (80304814)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 歯髄幹細胞 / 肝再生医療 / SHED

Outline of Final Research Achievements

We have developed a manufacturing process for isolating, culturing, and propagating multi-colony-derived SHEDs under XF/SF conditions, as well as a system for storing cells in two stages, MCB and WCB. We established quality control for the safety and quality (population doubling, chromosome stability, microbiological safety, and stem cell characteristics) of SHEDs as final products and SHEDs stored in MCBs and WCBs. In addition, we established a system to evaluate the in vivo immunogenicity and tumorigenicity of SHEDs as final products and stored products, as well as their liver regeneration effects using a mouse model of carbon tetrachloride-induced chronic liver fibrosis.

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Free Research Field

小児外科、肝臓移植、再生医療

Academic Significance and Societal Importance of the Research Achievements

本研究でおこなった製造と品質検査によって得られたSHED最終製品は、慢性肝線維症の治療のために安全で有効な治療成績が示された。