Development of a Novel Lung Tumor Marking Method Utilizing Fluorescence Spectrum Analysis Thoracoscopy and 5-ALA

2023 Fiscal Year Final Research Report

• Project/Area Number: 18K08774
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55040:Respiratory surgery-related
• Research Institution: Hokkaido University
• Principal Investigator: Aragaki Masato 北海道大学, 大学病院, 講師 (30788245)
• Co-Investigator(Kenkyū-buntansha): 加藤 達哉 北海道大学, 大学病院, 教授 (20624232) ; 海老原 裕磨 北海道大学, 大学病院, 特任教授 (50632981) ; 櫻井 遊 千葉大学, 大学院薬学研究院, 特任助教 (00707234) ; 樋田 泰浩 北海道大学, 大学病院, 准教授 (30399919) ; 加賀 基知三 北海道大学, 大学病院, 講師 (80224335)
• Project Period (FY): 2022-01-04 – 2024-03-31
• Keywords: 胸腔鏡手術 / 肺がん / 光線力学的治療

Outline of Final Research Achievements

This study aimed to develop a new marking method for thoracoscopic surgery targeting lung tumors. We used 5-ALA, porphyrin-based porphyrin lipoprotein (PLP), Porphysome, and OTL38. While 5-ALA proved difficult for marking lung tumors due to its short excitation light, other photosensitizers showed potential applicability. The efficacy of these methods was tested using mouse and porcine pseudo-tumor models, and the potential for clinical application was discussed. Additionally, we presented basic data on sentinel lymph node mapping for lung cancer using ICG. The application of photosensitizers in lung cancer surgery and treatment extends to tumor identification and photodynamic therapy (PDT). Specifically, we found that PLP-based PDT exhibited high anti-tumor effects and induced an abscopal effect, and these findings were published.

Free Research Field

呼吸器外科

Academic Significance and Societal Importance of the Research Achievements

胸腔鏡手術は、術後の早期回復、除痛効果、美容上の利点など、多くの利点を持つ低侵襲手術であり、機能温存による術後患者のQOL向上にもつながる。精度の高い腫瘍局在診断法が確立すれば、低侵襲手術の確立、機能温存、手術時間の短縮、術後在院日数の短縮、術後障害の軽減によるQOLの向上、そして確実な腫瘍の切除が期待できる。また、PDTが肺癌治療の選択肢の一つとなれば、低侵襲な治療としてその意義は大きい。