Establishment of antibody-cocktail therapy against invading bacteria for sepsis patients

2021 Fiscal Year Final Research Report

• Project/Area Number: 18K08868
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55050:Anesthesiology-related
• Research Institution: Tokai University
• Principal Investigator: Watanabe Nobuo 東海大学, 医学部, 助教 (80396928)
• Co-Investigator(Kenkyū-buntansha): 井上 茂亮 東海大学, 医学部, 准教授 (30582209)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Keywords: モノクローナル抗体 / 感染症 / 薬剤耐性 / 活性抗体

Outline of Final Research Achievements

Establishment of a therapeutic strategy against infectious diseases caused by drug-resistant bacteria including Methicillin-Resistant Staphylococcus aureus (MRSA) is a long-standing challenge; however, almost no antibody to MRSA has successfully showed effectiveness in the clinical trials. In this study, we targeted 2 master proteins responsible for toxin synthesis and 3 key proteins responsible for cell wall biosynthesis, respectively, and generated a total of 25 monoclonal antibodies in mice. Unfortunately, however, none of them demonstrated expected anti-MRSA activity on its own. However, now it has become possible to assess the anti-MRSA effect of these antibodies in combination with other antibodies and antibiotics etc.

Free Research Field

感染症

Academic Significance and Societal Importance of the Research Achievements

本研究において作製した抗MRSA抗体はいずれも単独では当初期待した効果(抗毒素産生作用ならびに抗増殖作用)は見られなかった。しかしこの結果、5種のタンパク質に関しては、今後の標的とすべき配列が絞り込まれたことになる。また、今後は他の抗体ならびに各種の抗菌剤との併用効果を検証しすることによって付加的な抗菌効果が得られるか検討する予定である。ハイブリドーマは樹立され液体窒素に保管されているため、必要に応じてすぐに抗体の追加産生ならびにヒト化抗体化が可能である。