Study on the physiological and pathological function of 8-nitro-cGMP in bone remodeling.

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K09513
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57010:Oral biological science-related
• Research Institution: Showa University
• Principal Investigator: Miyamoto Yoichi 昭和大学, 歯学部, 准教授 (20295132)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 骨リモデリング / 破骨細胞 / 骨芽細胞 / 一酸化窒素 / 活性酸素種

Outline of Final Research Achievements

Bones' shape and mechanical strength are maintained through bone remodeling composed of bone resorption by osteoclasts and bone formation by osteoblasts. The roles of nitric oxide (NO) and reactive oxygen species (ROS) in bone remodeling have not been fully clarified. In this study, we examined the production and function of 8-nitro-cGMP, a novel signaling molecule of NO and ROS, in osteoclasts, osteoblasts, and osteocytes. All the three types of cells examined produced 8-nitro-cGMP. 8-nitro-cGMP promoted osteoclast differentiation and suppressed mineralization by osteoblasts. These results indicate that 8-nitro-cGMP is a regulator that supports bone resorption and suppresses bone formation.

Free Research Field

生化学

Academic Significance and Societal Importance of the Research Achievements

8-ニトロ-cGMPが骨吸収を促進し、骨形成を抑制することが示唆された。生体内での8-nitro-cGMPの生成経路、シグナル伝達様式および不活性化経路は、すでに明らかになっている。骨粗鬆症では、骨吸収が骨形成に対して優位になることで骨量が減少する。8-ニトロ-cGMPの生成を抑制あるいは不活性化を促進することで、骨吸収を抑制し、骨形成を促進できれば、骨粗鬆症の病態を改善できる可能性がある。