Bone remodeling via RANL/OPG intracellular transportation

2021 Fiscal Year Final Research Report

• Project/Area Number: 18K09516
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57010:Oral biological science-related
• Research Institution: Osaka Dental University
• Principal Investigator: Notomi Takuya 大阪歯科大学, 歯学部, 講師 (70542249)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Keywords: 破骨細胞 / OPG / 光遺伝学

Outline of Final Research Achievements

The investigation for bone diseases was developed mainly via suppression of bone resorption including RANKL intracellular signaling. However, the application for OPG which is a RANKL-decoy receptor are not well examined. In osteoblasts expressing RANKL-venus or OPG-cherry, long-term stretch-mechanical stimulus induced the distinct intracellular transportation. Long-term light stimulus which induces the changes in membrane potential promoted the intracellular transportation of OPG but not RANKL, suggesting that specific condition of light stimulus or mechanical stimulus would occur the distinct intracellular transportation of OPG.

Free Research Field

骨生物学

Academic Significance and Societal Importance of the Research Achievements

本研究では、RANKLとOPGの細胞内輸送経路に焦点を当てて検討した。従来の研究では、RANKLについての輸送経路についての検討が行われてきたが、OPGについては不明確であった。本研究で明らかになった、RANKLとOPGの細胞内輸送経路の違いは、破骨細胞形成と骨芽細胞機能に関わる分子メカニズムの新知見であり、骨量回復のための、骨粗鬆症治療薬の新たな標的につながる。