Effects of masticatory function on extracellular matrix expression and mechanical property of the TMJ disc

2020 Fiscal Year Annual Research Report

• Project/Area Number: 18K09862
• Research Institution: Tohoku University
• Principal Investigator: 溝口 到 東北大学, 歯学研究科, 教授 (20200032)
• Co-Investigator(Kenkyū-buntansha): 飯嶋 雅弘 北海道医療大学, 歯学部, 教授 (20305915) ; 鳥谷 奈保子 北海道医療大学, 歯学部, 助教 (20433435) [Withdrawn] ; 岡山 三紀 北海道医療大学, 歯学部, 講師 (30382500) ; 荒川 俊哉 北海道医療大学, 歯学部, 教授 (40306254)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 関節円板 / 細胞外基質 / コラーゲン / プロテオグリカン / 軟食摂取

Outline of Annual Research Achievements

顎関節を構成する主要な細胞外マトリックスは、組織の形態維持、機械的特性、脆弱性および顎関節症などの病的変化において重要な役割を担っている。本研究では、7週齢Wistarラットの切歯部バイトプレートを用いて顎関節の関節荷重を増大させる実験モデルを用い、両群の関節円板について円板組織の機械的特性に関わる主要な細胞外マトリックスであるプロテオグリカン、Ⅰ型、Ⅱ型およびⅢ型コラーゲンの局在と発現にどのような変化が生じるのかについて検討した。
その結果、以下のことが明らかになった。
(1) 2週以降、関節円板後方肥厚部での厚径の増大とトルイジンブルー染色の染色性の亢進（グリコサミノグリカンの集積）がみられた。このグリコサミノグリカンの集積は、関節円板の後方肥厚部に限局しており、この領域が切歯咬合時での機能力の負荷部位であることを示唆している。(2) 顎関節への過重負荷の増大によってプロテオグリカンでは挙上2週でversicanとbiglycanのmRNA発現が亢進し、3週目でdecorinのmRNA発現が亢進した。(3) コラーゲンでは1週後にⅠ型コラーゲンのmRNA発現が減少し、Ⅲ型コラーゲンのmRNA発現が亢進した。3週以降はⅠ型コラーゲンのmRNA発現が亢進し、Ⅲ型コラーゲンのmRNA発現が減少した。Ⅱ型コラーゲンの発現の変化はみられなかった。
以上の結果から、咬合改変に伴う関節荷重の増大は、関節円板の細胞外基質のmRNA発現に影響を及ぼすことが明らかになった。今後は、今まで得られた知見をもとに、さらに円板の細胞外基質のmRNA発現の領域差について検討していく必要がある。

Research Products

• [Journal Article] The miR-155-5p inhibits osteoclast differentiation through targeting CXCR2 in orthodontic root resorption (2021)
  • Author(s): Jiang H, Kitaura H, Liu L, Mizoguchi I, Liu S.
  • Journal Title: J Periodontal Res Volume: - Pages: -
  • DOI: 10.1111/jre.12875.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Inhibition of the CXCL9-CXCR3 axis suppresses the progression of experimental apical periodontitis by blocking macrophage migration and activation (2021)
  • Author(s): Hasegawa T, Venkata Suresh V, Yahata Y, Nakano M, Suzuki S, Suzuki S, Yamada S, Kitaura H, Mizoguchi I, Noiri Y, Handa K, Saito M
  • Journal Title: Sci Rep Volume: 11 Pages: 2613
  • DOI: 10.1038/s41598-021-82167-7.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Local administration of high-dose diabetes medicine exendin-4 inhibits orthodontic tooth movement in mice (2021)
  • Author(s): Shen WR, Kitaura H, Qi J, Ogawa S, Ohori F, Noguchi T, Marahleh A, Nara Y, Adya P, Mizoguchi I.
  • Journal Title: Angle Orthod Volume: 91 Pages: 111-118
  • DOI: 10.2319/021320-103.1.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] TNF-α stimulates the expression of RANK during orthodontic tooth movement. (2020)
  • Author(s): Noguchi T, Kitaura H, Ogawa S, Qi J, Shen W-R, Ohori F, Marahleh A, Nara Y, Pramusita A, Mizoguchi I
  • Journal Title: Archives of Oral Biology Volume: 117 Pages: 104796
  • DOI: 10.1016/j.archoralbio.2020.104796.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Anti-c-fms antibody prevents osteoclast formation and bone resorption in co-culture of osteoblasts and osteoclast precursors in vitro and in ovariectomized mice. (2020)
  • Author(s): Nara Y, Kitaura H, Ogawa S, Shen W-R, Qi J, Ohori F, Noguchi T, Marahleh A, Pramusita A, Kinjo R, Itaru Mizoguchi I
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Pages: 6120
  • DOI: 10.3390/ijms21176120.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effects of bioactive glass coating by electrophoretic deposition on esthetical, bending, and frictional performance of orthodontic stainless steel wire. (2020)
  • Author(s): Kawaguchi K, Iijima M, Muguruma T, Endo K, Mizoguchi I
  • Journal Title: Dental Materials Journal Volume: 39 Pages: 593 - 600
  • DOI: 10.4012/dmj.2019-085.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Osteocyte-related cytokines regulate osteoclast formation and bone resorption. (2020)
  • Author(s): Kitaura H, Marahleh A, Ohori F, Noguchi T, Shen WR, Qi J, Nara Y, Pramusita A, Kinjo R, Mizoguchi I
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Pages: 5169
  • DOI: 10.3390/ijms21145169.
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effect of a DPP-4 inhibitor on orthodontic tooth movement and associated root resorption. (2020)
  • Author(s): Qi J, Kitaura H, Shen WR, Ogawa S, Ohori F, Noguchi T, Marahleh A, Nara Y, Adya P, Mizoguchi I
  • Journal Title: Biomed Res Int Volume: 2020 Pages: 7189084
  • DOI: 10.1155/2020/7189084.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Obtaining Primary Osteocytes through Murine Calvarial Fractionation of GFP-Expressing Osteocytes (2020)
  • Author(s): Marahleh A, Kitaura H, Ogawa S, Shen WR, Qi J, Ohori F, Noguchi T, Nara Y, Pramusita A, Kinjo R, Mizoguchi I.
  • Journal Title: J Vis Exp Volume: 160 Pages: -
  • DOI: 10.3791/61513.
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sugar Metabolism of Scardovia wiggsiae, a Novel Caries-Associated Bacterium (2020)
  • Author(s): Kameda M, Abiko Y, Washio J, Tanner ACR, Kressirer CA, Mizoguchi I, Takahashi N.
  • Journal Title: Front Microbiol Volume: 11 Pages: 479
  • DOI: 10.3389/fmicb.2020.00479.
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Inhibitory Effect of IL-33 on TNF-α-Induced Osteoclastogenesis and Orthodontic Tooth Movement. (2020)
  • Author(s): Ohori F, Kitaura H, Ogawa S, Noguchi T, Marahleh A, Nara Y, Pramusita A, Kinjo R, Mizoguchi I
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] Diabetes Medicine Glucagon-Like Peptode-1 Receptor Agonist Inhibits Orthodontic Tooth Movement and Root Resorption. (2020)
  • Author(s): Ohori F, Kitaura H, Shen WR, Ogawa S, Noguchi T, Marahleh A, Nara Y, Pramusita A, Kinjo R, Mizoguchi I
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] Diabetes medicine DPP-4 inhibitor inhibits orthodontic tooth movement and associated root resorption in mouse tooth movement model. (2020)
  • Author(s): Noguchi T, Kitaura H, Qi J, Ogawa S, Ohori F, Marahleh A, Nara Y, Pramusita A, Kinjo R, Mizoguchi I
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] TNF-α stimulates the expression of RANK during orthodontic tooth movement. (2020)
  • Author(s): Noguchi T, Kitaura H, Ogawa S, Ohori F, Marahleh A, Nara Y, Pramusita A, Kinjo R, Mizoguchi I
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] Role of TNF-α-responsible stromal clls to osteoclast and odontoclast formation during orthodontic tooth movement. (2020)
  • Author(s): Ogawa S, Kitaura H, Ohori F, Noguchi T, Marahleh A, Nara Y, Pramusita A, Mizoguchi I
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] Statistical and clinical survey of congenital disease patients in the past 10-year period in Tohoku University Hospital Department of orthodontics. (2020)
  • Author(s): Sasaki S, Kitaura H, Maeda T, Ogawa S, Ogura H, Kameda M, Kinjo R, Shigemi S, Numazaki K, Mizoguchi I
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] Craniofacial characteristics, malocclusions and successful orthodontic treatment results in 3 Diamond Blackfan Syndrome patients. (2020)
  • Author(s): Sasaki S, Kitaura H, Yoshida M, Mayama A, Mizoguchi I
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] Micro-osteoperforations induce TNF-α expression and accelerate orthodontic tooth movement in mice. (2020)
  • Author(s): Kinjo R, Kitaura H, Ogawa S, Ohori F, Noguchi T, Marahleh A, Nara Y, Pramusita A, Mizoguchi I.
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] Solt-sensitive hypertension exacerbates osteoclastogenesis and bone resorption in vivo. (2020)
  • Author(s): Pramusita A, Kitaura H, Ogawa S, Ohori F, Noguchi T, Marahleh A, Nara Y, Kinjo R, Mizoguchi I
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] TNF-α induces orthodontic tooth movement and enhances osteocyte RANKL expression by activating MAPKs and NFκB pathway. (2020)
  • Author(s): Marahleh A, Kitaura H, Ohori F, Ogawa S, Noguchi T, Nara Y, Pramusita A, Kinjo R, Mizoguchi I.
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] Facial asymmetry and unilateral condylar hyperplasia treated with ipsilateral condylectomy and orthodontic treatment. (2020)
  • Author(s): Kishikawa A, Fukunaga T, Nogami S, Seiryu M, Shigemi S, Kumamoto H, Takahashi T, Mizoguchi I
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] Three-dimensional evaluation of temporomandibular joint cavity in three cases with severe skeletal asymmetry and occlusal cant. (2020)
  • Author(s): Oyanagi T, Maeda T, Yoshizawa M, Kimura S, Mayama A, Seiryu M, Mizoguchi I
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] Treatment of open bite and unilateral scissors bite with orthodontic anchor screws in a middle-age (2020)
  • Author(s): Oyanagi T, Yoshida M, Mayama A, Masahiro Seiryu M, Itaru Mizoguchi
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] Five-year clinical investigation of orthodontic anchow screw used for surgical orthodontic treatment (2020)
  • Author(s): Sasaki S, Mayama A, Oyangi T, Ito A, Ogura H, Seiryu M, Mizoguchi I
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] 3D evaluation of changes in the alveolar bone due to upper anterior teeth retraction with orthodontic anchor screws. (2020)
  • Author(s): Ito A, Mayama A, Oyanagi T, Sasaki S, Ogura H, Seiryu M, Mizoguchi I
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] 3D cast analysis of 10 years retention case treated with miniscrews for distalization of maxillary molar. (2020)
  • Author(s): Irie D, Oyanagi T, Ito-Okubo M, Mayama A, Seiryu M, Mizoguchi I
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research