The role of circadian abnormalities in salivary gland disorders

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K09922
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57080:Social dentistry-related
• Research Institution: Nihon University
• Principal Investigator: BHAWAL Ujjal 日本大学, 松戸歯学部, 講師 (50433339)
• Co-Investigator(Kenkyū-buntansha): 田中 ゆり子 東邦大学, 医学部, 講師 (40396685) ; 佐藤 冬樹 静岡県立静岡がんセンター(研究所), その他部局等, 研究員 (60400131)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 加齢 / 唾液腺 / DNAマイクロアレイ / microRNAアレイ

Outline of Final Research Achievements

Submandibular glands of 3-month-old and 24-month-old C57BL/6 mice were used for analysis of genes and miRNAs using DNA microarrays and miRNA arrays in combination with Gene Spring and Ingenuity Pathways Analysis. The regulatory transcription factors of aging are involved in the regulation of miR-30c-1-3p, miR-34a-5p, miR-92a-3p, miR-181a-5p, and miR-550a-3p. The 3'UTR activity of the DEC1 was increased with aging but it was alleviated in the mutation of the target miRNA binding site in the luciferase reporter assay. In addition, DEC1 expression plasmid was forcibly suppressed the E-cadherin transcription activity in a dose-dependent manner. Furthermore, Chromatin immunoprecipitation (ChIP assay) confirmed that DEC1 regulates E-cadherin. Collectively, our data suggest a regulatory effect of DEC1 in E-cadherin-mediated salivary gland function.

Free Research Field

医歯薬学

Academic Significance and Societal Importance of the Research Achievements

高齢化に伴う唾液分泌障害者の増加は歯周病のリスクを上げるだけではなく、嚥下障害や誤嚥性肺炎の発症を招くなど、全身にも悪影響を与える。唾液腺樹状細胞が唾液腺健康維持に重要な働きをしていることが明らかになり、シェーグレン症候群や加齢など唾液腺疾患の発症機序の解明にも大きく貢献することが期待されている。本研究において、唾液腺の老化のメカニズムを解明するだけでなく、唾液分泌障害の診断や対処法の確立に役立つと思われる。