Investigation of serum fatty acid amides as useful biomarkers

2020 Fiscal Year Final Research Report

• Project/Area Number: 18K11018
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 59040:Nutrition science and health science-related
• Research Institution: Nagoya University
• Principal Investigator: Yoshitake Jun 名古屋大学, 未来社会創造機構, 特任助教 (70414349)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 血中脂肪酸アミド濃度 / オレアミド / 抗炎症作用

Outline of Final Research Achievements

To quantify the fatty acid amides acting as a lipid mediator with high sensitivity, a quantification method that uses mass spectrometry coupled with a stable isotope dilution method was established. When quantify the fatty acid amides in mice, we observed reduced oleamide concentration of serum in LPS-induced acute inflammatory model mice. The administration of oleamide to the model mice decreased inflammatory cytokines in serum and increased survival rate. These results and the observation that the pre-treatment of the cells with oleamide reduced the production of pro-inflammatory cytokines by LPS stimulation suggest that oleamide has a possibility to exert anti-inflammatory effect.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

これまで脂肪酸アミドの脂質メディエーターとしての役割についての研究は多く行われている。今後恒常性維持の観点から、生体局所における脂肪酸アミドの定量や動向が重要となることが予想できる。本研究により構築した脂肪酸アミド類の高感度な定量法は、各種疾患と脂肪酸アミド類の相関の網羅的な解析や食品の機能性評価など、多くの分野への応用が期待できる。