2020 Fiscal Year Final Research Report
Elucidation of radiation-induced mammary carcinogenic mechanism by capturing spatiotemporal dynamics of mammary stem cells
Project/Area Number |
18K11652
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 63020:Radiation influence-related
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Research Institution | National Institutes for Quantum and Radiological Science and Technology |
Principal Investigator |
IIZUKA Daisuke 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 放射線影響研究部, 研究統括(定常) (00455388)
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Keywords | 放射線発がん / 乳腺 / 幹細胞 / 三次元免疫組織学的解析 / 細胞系譜追跡 |
Outline of Final Research Achievements |
Carcinogenic risk from low-dose radiation exposure is an important issue that must be scientifically clarified, and stem cells are considered to be the origin of radiation carcinogenesis. Therefore, in this study, we set up a cell lineage tracing experiment that can trace stem cells and their progenies and a three-dimensional immunohistochemical analysis in combination with tissue clearing. Using these techniques, we observed relatively early cell dynamics after radiation exposure. We could evaluate the presence of labeled stem cells and whether they were proliferating or not. This suggests the possibility of observing the effects of radiation exposure on mammary stem cells.
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Free Research Field |
放射線生物学
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Academic Significance and Societal Importance of the Research Achievements |
本研究では放射線誘発乳がんメカニズムを明らかにするために、放射線発がんの起源細胞と考えられる幹細胞に着目し、解析するための技術を導入した。これにより、放射線被ばくしてがんに至る幹細胞の動態を動物実験で明らかにすることが可能となった。将来的には福島原発事故で露呈した、100 mGy以下の被ばくによる発がんリスクを科学的に明らかにすることができると考えられる。
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