2018 Fiscal Year Research-status Report
Effects of antibiotics in wastewater on the removal of human enteric viruses by specific interaction between virus and wastewater solids
| Project/Area Number |
18K13855
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2020-03-31
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| Keywords | enteric virus / ARB / ARG / Specific interaction |
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| Outline of Annual Research Achievements |
Based on the minimum inhibitory value of amikacin and chloramphenicol to the E.cloacae SENG-6, a study was conducted to evaluate the EPS production under sub-inhibitory level antibiotics exposure.Soluble microbial products (SMP), lightly bound extracellular polymeric substances (LB-EPS) and tightly bound EPS (TB-EPS) were extracted and quantity of carbohydrates and proteins were measured. Among the three fractions, highest protein concentration was present in SMP whereas the highest carbohydrate concentration was present in the LB-EPS fraction. SDS-PAGE confirmed that the protein concentrations reduced with increased antibiotic concentration. By using western blot, it was confirmed that A-type HBGA concentration in the EPS was also reduced with increased antibiotic concentration.
A review paper titled "Understanding human health risks caused by antibiotic resistant bacteria (ARB) and resistance genes (ARG) in water environments: Questions to be answered" is under review in "Critical Reviews in Environmental Science and Technology".
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
The study is going as planned.
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| Strategy for Future Research Activity |
Excitation emission matrix (EEM) fluorescence spectroscopy on extracted bacterial EPS fractions are to be performed. It can reveal the differences between extracted EPS fractions from E.cloacae SENG-6 cultivated under different antibiotic concentrations. A rotavirus HAL1166 microfiltration test will be conducted in the presence of E.cloacae SENG-6 cultivated under different antibiotic concentrations. It is expected that composition changes in the EPS caused by exposing to antibiotics will affect the specific interactions between rotavirus HAL1166 and E.cloacae SENG-6 and thus different virus removal capabilities. A cross-flow membrane filtration system will be used to scale-up the experiment and observe the gel/cake layer properties.
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