Effects of antibiotics in wastewater on the removal of human enteric viruses by specific interaction between virus and wastewater solids

2019 Fiscal Year Final Research Report

• Project/Area Number: 18K13855
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 22060:Environmental systems for civil engineering-related
• Research Institution: Tohoku University
• Principal Investigator: Amarasiri Mohan 東北大学, 工学研究科, 助教 (50815537)
• Project Period (FY): 2018-04-01 – 2020-03-31
• Keywords: Enteric virus / ARB / ARG / Specific interaction

Outline of Final Research Achievements

We evaluated the MIC of amikacin and chloramphenicol to Enterobacter cloacae using E-test. After that we cultivated the bacteria under subinhibitory level antibiotics concentrations. We extracted the SMP, LB-EPS and TB-EPS production. Carbohydrate and protein concentrations of extracted fractions were measured. We observed changes in the EPS structure by Excitation Emission Matrix fluorescence spectroscopy. After that we conducted a microfiltration experiment of rotavirus HAL1166 in the presence of E.cloacae grown under different antibiotic concentrations.
A review paper titled "Understanding human health risks caused by antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in
water environments: Current knowledge and questions to be answered" is published in Critical Reviews in Environmental Science and Technology journal.

Free Research Field

土木環境システム関連

Academic Significance and Societal Importance of the Research Achievements

副抑制薬剤濃度の存在下で、細菌細胞のEPS生産が増加しました。細菌、ウイルス、薬剤がリアクター内に共存しているため、EPS濃度の増加はウイルスの付着の増加を引き起こす可能性があります。ただし、EPSの量が増えると、膜反応器にファウリングが発生する可能性があります。したがって、ウイルスの付着を増やし、ファウリングを減らすための最適な条件を特定することが重要です。