2020 Fiscal Year Final Research Report
Development of gene transfer method by laser-induced convergent pressure wave using annular beam
Project/Area Number |
18K14142
|
Research Category |
Grant-in-Aid for Early-Career Scientists
|
Allocation Type | Multi-year Fund |
Review Section |
Basic Section 30020:Optical engineering and photon science-related
|
Research Institution | Utsunomiya University |
Principal Investigator |
|
Project Period (FY) |
2018-04-01 – 2021-03-31
|
Keywords | 環状レーザービームの設計 / 計算機ホログラム / 細胞の表面位置の検出 / 超解像ビーム生成 / AIによる補償光学 |
Outline of Final Research Achievements |
The aim of this study is to develop a safe and highly efficient external gene transfer method to a single cell. In particular, we aimed to develop a more efficient gene transfer method than conventional methods by using convergent pressure waves induced by laser irradiation with an annular beam pattern. To achieve this goal, we designed an annular laser beam generated by a computer hologram. To achieve this goal, we designed an annular laser beam generated by a computer-generated holograms, and incorporated a confocal microscope into the optical system to measure the position of the cell surface for precise control of the beam irradiation position. In the course of our research, we invented a super-resolution beam irradiation method with higher spatial resolution. In addition, we demonstrated adaptive optics using artificial intelligence that enables high-quality beam irradiation even when the optical system is under aberration.
|
Free Research Field |
超短パルスレーザー加工,計算機ホログラム,光計測,光情報処理,AI光学
|
Academic Significance and Societal Importance of the Research Achievements |
現在, ポストゲノム研究として,ヒト遺伝子の個々の機能を解明するために,各遺伝子を生細胞に導入して発現を観測する手法が用いられている.その遺伝子導入法には,既に幾つかの手法が提案されているが,現状ではいずれの手法においても,標的細胞に安全で正確に,かつ高効率に遺伝子を導入する技術が確立されていない.また,膨大な数ある遺伝子の機能を選別する必要があり,多大な時間と労力を要するため,従来法に変わる高速なスクリーニング技術が有望されている.本研究では,非接触で安全なレーザーを用いた従来の遺伝子導入法をベースに,従来法が抱える課題を克服するための環状ビームを用いた新手法の開発を行った.
|